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Mouse CCR2 APC-conjugated Antibody

Catalog #: FAB5538A
35 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5538A-025
FAB5538A-100
Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry.
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Product Details
Citations (35)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR2 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 475301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
L1.2 mouse pro-B cell line transfected with mouse CCR2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR2 antibody in L1.2 Mouse Cell Line Transfected with Mouse CCR2 antibody by Flow Cytometry. View Larger

Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry. L1.2 mouse pro-B cell line transfected with mouse CCR2 was stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citations for Mouse CCR2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Authors: AE Geller, R Shrestha, MR Woeste, H Guo, X Hu, C Ding, K Andreeva, JH Chariker, M Zhou, D Tieri, CT Watson, RA Mitchell, HG Zhang, Y Li, RCG Martin Ii, EC Rouchka, J Yan
    Nature Communications, 2022-02-09;13(1):759.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice
    Authors: M Le Roux, A Ollivier, G Kervoaze, T Beke, L Gillet, M Pichavant, P Gosset
    Biomedicines, 2021-12-05;9(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Early activation of the cardiac CX3CL1/CX3CR1 axis delays beta-adrenergic-induced heart failure
    Authors: M Flamant, N Mougenot, E Balse, L Le Fèvre, F Atassi, EL Gautier, W Le Goff, M Keck, S Nadaud, C Combadière, A Boissonnas, C Pavoine
    Scientific Reports, 2021-09-09;11(1):17982.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Inhibition of the canonical Wnt signaling pathway by a beta-catenin/CBP inhibitor prevents heart failure by ameliorating cardiac hypertrophy and fibrosis
    Authors: T Methatham, S Tomida, N Kimura, Y Imai, K Aizawa
    Scientific Reports, 2021-07-21;11(1):14886.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The CCR2/MCP-1 Chemokine Pathway and Lung Adenocarcinoma
    Authors: P Mittal, L Wang, T Akimova, CA Leach, JC Clemente, MR Sender, Y Chen, BJ Turunen, WW Hancock
    Cancers, 2020-12-11;12(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Macrophage phenotypic switch orchestrates the inflammation and repair/regeneration following acute pancreatitis injury
    Authors: J Wu, L Zhang, J Shi, R He, W Yang, A Habtezion, N Niu, P Lu, J Xue
    EBioMedicine, 2020-07-30;58(0):102920.
    Species: Mouse
    Sample Types: Whole Cells
  7. Low-dose oral cyclophosphamide therapy reduces atherosclerosis progression by decreasing inflammatory cells in a murine model of atherosclerosis
    Authors: Y Sato-Okaba, K Isoda, B Heissig, T Kadoguchi, K Akita, K Kitamura, K Shimada, K Hattori, H Daida
    Int J Cardiol Heart Vasc, 2020-05-10;28(0):100529.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection
    Authors: SR Jung, TM Ashhurst, PK West, B Viengkhou, NJC King, IL Campbell, MJ Hofer
    PLoS Pathog., 2020-04-20;16(4):e1008525.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Csf1r or Mer inhibition delays liver regeneration via suppression of Kupffer cells
    Authors: JA Santamaria, S Zeng, JB Greer, MJ Beckman, AM Seifert, NA Cohen, JQ Zhang, MH Crawley, BL Green, JK Loo, JH Maltbaek, RP DeMatteo
    PLoS ONE, 2019-05-01;14(5):e0216275.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis
    Authors: J Li, S Wada, LK Weaver, C Biswas, EM Behrens, Z Arany
    JCI Insight, 2019-03-07;5(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. ISRE-Reporter Mouse Reveals High Basal and Induced Type I IFN Responses in Inflammatory Monocytes
    Authors: MB Uccellini, A García-Sas
    Cell Rep, 2018-12-04;25(10):2784-2796.e3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae
    Authors: F Madouri, O Barada, G Kervoaze, F Trottein, M Pichavant, P Gosset
    EBioMedicine, 2018-10-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?
    Authors: HM Hsiao, R Fernandez, S Tanaka, W Li, JH Spahn, S Chiu, M Akbarpour, D Ruiz-Perez, Q Wu, C Turam, D Scozzi, T Takahashi, HP Luehmann, V Puri, GRS Budinger, AS Krupnick, AV Misharin, KJ Lavine, Y Liu, AE Gelman, A Bharat, D Kreisel
    J. Clin. Invest., 2018-05-21;128(7):2833-2847.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. SOCS3 deficiency in myeloid cells promotes retinal degeneration and angiogenesis through arginase-1 up-regulation in experimental autoimmune uveoretinitis
    Authors: M Chen, J Zhao, IH Ali, S Marry, J Augustine, M Bhuckory, A Lynch, A Kissenpfen, H Xu
    Am. J. Pathol., 2018-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration
    Authors: CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick
    Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):662-673.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017-02-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
    Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
    Mucosal Immunol, 2017-01-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  18. Impact of myeloid-derived suppressor cell on Kupffer cells from mouse livers with hepatocellular carcinoma
    Authors: Stéphanie Lacotte
    Oncoimmunology, 2016-09-20;5(11):e1234565.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Pivotal role for skin trans-endothelial radio-resistant anti-inflammatory macrophages in tissue repair
    Authors: Olga Barreiro
    Elife, 2016-06-15;5(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  20. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Monocyte Trafficking, Engraftment, and Delivery of Nanoparticles and an Exogenous Gene into the Acutely Inflamed Brain Tissue - Evaluations on Monocyte-Based Delivery System for the Central Nervous System
    PLoS ONE, 2016-04-26;11(4):e0154022.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Donor colonic CD103+ dendritic cells determine the severity of acute graft-versus-host disease.
    Authors: Koyama M, Cheong M, Markey K, Gartlan K, Kuns R, Locke K, Lineburg K, Teal B, Leveque-El Mouttie L, Bunting M, Vuckovic S, Zhang P, Teng M, Varelias A, Tey S, Wockner L, Engwerda C, Smyth M, Belz G, McColl S, MacDonald K, Hill G
    J Exp Med, 2015-07-13;212(8):1303-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling.
    Authors: Patel, Jyoti, McNeill, Eileen, Douglas, Gillian, Hale, Ashley B, de Bono, Joseph, Lee, Regent, Iqbal, Asif J, Regan-Komito, Daniel, Stylianou, Elena, Greaves, David R, Channon, Keith M
    Nat Commun, 2015-03-18;6(0):6614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis.
    Authors: Watson N, Schneider K, Massa P
    J Immunol, 2015-02-13;194(6):2796-809.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Vitamin D deficiency decreases survival of bacterial meningoencephalitis in mice.
    Authors: Djukic M, Sostmann N, Bertsch T, Mecke M, Nessler S, Manig A, Hanisch U, Triebel J, Bollheimer L, Sieber C, Nau R
    J Neuroinflammation, 2015-01-07;12(0):208.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  26. Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.
    Authors: Habbeddine M, Verbeke P, Karaz S, Bobe P, Kanellopoulos-Langevin C
    PLoS ONE, 2014-09-26;9(9):e107267.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Preemptive donor apoptotic cell infusions induce IFN-gamma-producing myeloid-derived suppressor cells for cardiac allograft protection.
    Authors: Bryant J, Lerret N, Wang J, Kang H, Tasch J, Zhang Z, Luo X
    J Immunol, 2014-05-07;192(12):6092-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Collective nitric oxide production provides tissue-wide immunity during Leishmania infection.
    Authors: Olekhnovitch R, Ryffel B, Muller A, Bousso P
    J Clin Invest, 2014-03-10;124(4):1711-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection.
    Authors: Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R
    J Neuroinflammation, 2014-01-23;11(0):14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. The monocyte to macrophage transition in the murine sterile wound.
    Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
    PLoS ONE, 2014-01-22;9(1):e86660.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Calcitonin gene-related peptide regulates type IV hypersensitivity through dendritic cell functions.
    Authors: Mikami, Norihisa, Sueda, Kaori, Ogitani, Yusuke, Otani, Ippei, Takatsuji, Miku, Wada, Yasuko, Watanabe, Keiko, Yoshikawa, Rintaro, Nishioka, Satoshi, Hashimoto, Nagisa, Miyagi, Yayoi, Fukada, So-ichir, Yamamoto, Hiroshi, Tsujikawa, Kazutake
    PLoS ONE, 2014-01-21;9(1):e86367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis.
    Authors: van Nieuwenhuijze A, Coghill E, Gray D, Prato S, Metcalf D, Alexander W, Wicks I
    Am J Pathol, 2013-10-30;184(1):184-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
    Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
    Mucosal Immunol, 2012-09-12;6(2):393-404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4.
    Authors: Schiraldi M, Raucci A, Munoz LM, Livoti E, Celona B, Venereau E, Apuzzo T, De Marchis F, Pedotti M, Bachi A, Thelen M, Varani L, Mellado M, Proudfoot A, Bianchi ME, Uguccioni M
    J. Exp. Med., 2012-02-27;209(3):551-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Calcitonin gene-related peptide is an important regulator of cutaneous immunity: effect on dendritic cell and T cell functions.
    J. Immunol., 2011-05-06;186(12):6886-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse CCR2 APC-conjugated Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Mouse CCR2 APC-conjugated Antibody
By Jim Hsiao on 05/23/2017
Application: Flow Sample Tested: Lung tissue Species: Mouse

Single cell suspensions of mouse cells were isolated from the lung tissue and were blocked with Fc block for 30 minutes. Cells were then stained with the APC-conjugated anti-mouse CCR2 antibody (1: 100 in FACS buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.
Data was pregated with FSC/SSC --> FSC-A/FSC-W

Flow Cytometry Mouse CCR2 APC-conjugated Antibody FAB5538A
Mouse CCR2 APC-conjugated Antibody
By Kihong Lim on 09/29/2016
Application: Flow Sample Tested: activated mouse CD8 T cell Species: Mouse

CCR2 expression on mouse CD8 T cell in vitro activated or virus-infected lung

Flow Cytometry Mouse CCR2 APC-conjugated Antibody FAB5538A
Mouse CCR2 APC-conjugated Antibody
By Anonymous on 08/24/2016
Application: Flow Sample Tested: Tumor cell lyastes Species: Mouse