Mouse CCR2 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5538A-025
FAB5538A-100
Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry.
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Product Details
Citations (54)
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Supplemental Products
Reviews (3)

Mouse CCR2 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 475301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
L1.2 mouse pro-B cell line transfected with mouse CCR2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR2 antibody in L1.2 Mouse Cell Line Transfected with Mouse CCR2 antibody by Flow Cytometry. View Larger

Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry. L1.2 mouse pro-B cell line transfected with mouse CCR2 was stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citations for Mouse CCR2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

54 Citations: Showing 1 - 10
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  1. Analysis of the Microglial Sensome
    Authors: Suzanne E. Hickman, Joseph El Khoury
    Methods Mol. Biol.
  2. Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes
    Authors: Sabine Spath, Florence Roan, Scott R. Presnell, Barbara Höllbacher, Steven F. Ziegler
    iScience
  3. PI3Kδ hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner
    Authors: Anne-Katrien Stark, Anita Chandra, Krishnendu Chakraborty, Rafeah Alam, Valentina Carbonaro, Jonathan Clark et al.
    Nature Communications
  4. Resident macrophages restrain pathological adipose tissue remodeling and protect vascular integrity in obese mice
    Authors: Qi Chen, Si Min Lai, Shaohai Xu, Yingrou Tan, Keith Leong, Dehua Liu et al.
    EMBO reports
  5. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins
    Authors: Lim J, Park H, Heisler J et al.
    Elife
  6. Regulation of age-related macular degeneration-like pathology by complement factor H
    Authors: Christopher B. Toomey, Una Kelly, Daniel R. Saban, Catherine Bowes Rickman
    Proceedings of the National Academy of Sciences
  7. CCR2 Signal Facilitates Thymic Egress by Priming Thymocyte Responses to Sphingosine-1-Phosphate
    Authors: Abudureyimujiang Aili, Jie Zhang, Jia Wu, Haoming Wu, Xiuyuan Sun, Qihua He et al.
    Frontiers in Immunology
  8. Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints
    Authors: M. E. Mikucki, D. T. Fisher, J. Matsuzaki, J. J. Skitzki, N. B. Gaulin, J. B. Muhitch et al.
    Nature Communications
  9. Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer
    Authors: Anders Etzerodt, Morgane Moulin, Thomas Koed Doktor, Marcello Delfini, Noushine Mossadegh-Keller, Marc Bajenoff et al.
    Journal of Experimental Medicine
  10. Macrophages promote anti-androgen resistance in prostate cancer bone disease
    Authors: Li XF, Selli C, Zhou HL et al.
    The Journal of experimental medicine
  11. Cytokine Deposition Alters Leukocyte Morphology and Initial Recruitment of Monocytes and gamma δT Cells After Corneal Injury
    Authors: Sonali Pal-Ghosh, Ahdeah Pajoohesh-Ganji, A. Sue Menko, Hye-young Oh, Gauri Tadvalkar, Daniel R. Saban et al.
    Investigative Opthalmology & Visual Science
  12. Effective-mononuclear cell (E-MNC) therapy alleviates salivary gland damage by suppressing lymphocyte infiltration in Sjögren-like disease
    Authors: Kayo Hasegawa, Jorge Luis Montenegro Raudales, Takashi I, Takako Yoshida, Ryo Honma, Mayumi Iwatake et al.
    Frontiers in Bioengineering and Biotechnology
  13. The impact of ranitidine on monocyte responses in the context of solid tumors
    Authors: Ava Vila-Leahey, Dakota Rogers, Jean S. Marshall
    Oncotarget
  14. The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression
    Authors: AE Geller, R Shrestha, MR Woeste, H Guo, X Hu, C Ding, K Andreeva, JH Chariker, M Zhou, D Tieri, CT Watson, RA Mitchell, HG Zhang, Y Li, RCG Martin Ii, EC Rouchka, J Yan
    Nature Communications, 2022-02-09;13(1):759.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice
    Authors: M Le Roux, A Ollivier, G Kervoaze, T Beke, L Gillet, M Pichavant, P Gosset
    Biomedicines, 2021-12-05;9(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Early activation of the cardiac CX3CL1/CX3CR1 axis delays beta-adrenergic-induced heart failure
    Authors: M Flamant, N Mougenot, E Balse, L Le Fèvre, F Atassi, EL Gautier, W Le Goff, M Keck, S Nadaud, C Combadière, A Boissonnas, C Pavoine
    Scientific Reports, 2021-09-09;11(1):17982.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Inhibition of the canonical Wnt signaling pathway by a beta-catenin/CBP inhibitor prevents heart failure by ameliorating cardiac hypertrophy and fibrosis
    Authors: T Methatham, S Tomida, N Kimura, Y Imai, K Aizawa
    Scientific Reports, 2021-07-21;11(1):14886.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. The CCR2/MCP-1 Chemokine Pathway and Lung Adenocarcinoma
    Authors: P Mittal, L Wang, T Akimova, CA Leach, JC Clemente, MR Sender, Y Chen, BJ Turunen, WW Hancock
    Cancers, 2020-12-11;12(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Macrophage phenotypic switch orchestrates the inflammation and repair/regeneration following acute pancreatitis injury
    Authors: J Wu, L Zhang, J Shi, R He, W Yang, A Habtezion, N Niu, P Lu, J Xue
    EBioMedicine, 2020-07-30;58(0):102920.
    Species: Mouse
    Sample Types: Whole Cells
  20. Low-dose oral cyclophosphamide therapy reduces atherosclerosis progression by decreasing inflammatory cells in a murine model of atherosclerosis
    Authors: Y Sato-Okaba, K Isoda, B Heissig, T Kadoguchi, K Akita, K Kitamura, K Shimada, K Hattori, H Daida
    Int J Cardiol Heart Vasc, 2020-05-10;28(0):100529.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection
    Authors: SR Jung, TM Ashhurst, PK West, B Viengkhou, NJC King, IL Campbell, MJ Hofer
    PLoS Pathog., 2020-04-20;16(4):e1008525.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Csf1r or Mer inhibition delays liver regeneration via suppression of Kupffer cells
    Authors: JA Santamaria, S Zeng, JB Greer, MJ Beckman, AM Seifert, NA Cohen, JQ Zhang, MH Crawley, BL Green, JK Loo, JH Maltbaek, RP DeMatteo
    PLoS ONE, 2019-05-01;14(5):e0216275.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis
    Authors: J Li, S Wada, LK Weaver, C Biswas, EM Behrens, Z Arany
    JCI Insight, 2019-03-07;5(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. ISRE-Reporter Mouse Reveals High Basal and Induced Type I IFN Responses in Inflammatory Monocytes
    Authors: MB Uccellini, A García-Sas
    Cell Rep, 2018-12-04;25(10):2784-2796.e3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae
    Authors: F Madouri, O Barada, G Kervoaze, F Trottein, M Pichavant, P Gosset
    EBioMedicine, 2018-10-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?
    Authors: HM Hsiao, R Fernandez, S Tanaka, W Li, JH Spahn, S Chiu, M Akbarpour, D Ruiz-Perez, Q Wu, C Turam, D Scozzi, T Takahashi, HP Luehmann, V Puri, GRS Budinger, AS Krupnick, AV Misharin, KJ Lavine, Y Liu, AE Gelman, A Bharat, D Kreisel
    J. Clin. Invest., 2018-05-21;128(7):2833-2847.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. SOCS3 deficiency in myeloid cells promotes retinal degeneration and angiogenesis through arginase-1 up-regulation in experimental autoimmune uveoretinitis
    Authors: M Chen, J Zhao, IH Ali, S Marry, J Augustine, M Bhuckory, A Lynch, A Kissenpfen, H Xu
    Am. J. Pathol., 2018-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration
    Authors: CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick
    Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):662-673.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017-02-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
    Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
    Mucosal Immunol, 2017-01-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  31. Impact of myeloid-derived suppressor cell on Kupffer cells from mouse livers with hepatocellular carcinoma
    Authors: Stéphanie Lacotte
    Oncoimmunology, 2016-09-20;5(11):e1234565.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Pivotal role for skin trans-endothelial radio-resistant anti-inflammatory macrophages in tissue repair
    Authors: Olga Barreiro
    Elife, 2016-06-15;5(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  33. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Monocyte Trafficking, Engraftment, and Delivery of Nanoparticles and an Exogenous Gene into the Acutely Inflamed Brain Tissue - Evaluations on Monocyte-Based Delivery System for the Central Nervous System
    PLoS ONE, 2016-04-26;11(4):e0154022.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Donor colonic CD103+ dendritic cells determine the severity of acute graft-versus-host disease.
    Authors: Koyama M, Cheong M, Markey K, Gartlan K, Kuns R, Locke K, Lineburg K, Teal B, Leveque-El Mouttie L, Bunting M, Vuckovic S, Zhang P, Teng M, Varelias A, Tey S, Wockner L, Engwerda C, Smyth M, Belz G, McColl S, MacDonald K, Hill G
    J Exp Med, 2015-07-13;212(8):1303-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling.
    Authors: Patel, Jyoti, McNeill, Eileen, Douglas, Gillian, Hale, Ashley B, de Bono, Joseph, Lee, Regent, Iqbal, Asif J, Regan-Komito, Daniel, Stylianou, Elena, Greaves, David R, Channon, Keith M
    Nat Commun, 2015-03-18;6(0):6614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis.
    Authors: Watson N, Schneider K, Massa P
    J Immunol, 2015-02-13;194(6):2796-809.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Vitamin D deficiency decreases survival of bacterial meningoencephalitis in mice.
    Authors: Djukic M, Sostmann N, Bertsch T, Mecke M, Nessler S, Manig A, Hanisch U, Triebel J, Bollheimer L, Sieber C, Nau R
    J Neuroinflammation, 2015-01-07;12(0):208.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  39. Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.
    Authors: Habbeddine M, Verbeke P, Karaz S, Bobe P, Kanellopoulos-Langevin C
    PLoS ONE, 2014-09-26;9(9):e107267.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. Preemptive donor apoptotic cell infusions induce IFN-gamma-producing myeloid-derived suppressor cells for cardiac allograft protection.
    Authors: Bryant J, Lerret N, Wang J, Kang H, Tasch J, Zhang Z, Luo X
    J Immunol, 2014-05-07;192(12):6092-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. Collective nitric oxide production provides tissue-wide immunity during Leishmania infection.
    Authors: Olekhnovitch R, Ryffel B, Muller A, Bousso P
    J Clin Invest, 2014-03-10;124(4):1711-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection.
    Authors: Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R
    J Neuroinflammation, 2014-01-23;11(0):14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  43. The monocyte to macrophage transition in the murine sterile wound.
    Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
    PLoS ONE, 2014-01-22;9(1):e86660.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  44. Calcitonin gene-related peptide regulates type IV hypersensitivity through dendritic cell functions.
    Authors: Mikami, Norihisa, Sueda, Kaori, Ogitani, Yusuke, Otani, Ippei, Takatsuji, Miku, Wada, Yasuko, Watanabe, Keiko, Yoshikawa, Rintaro, Nishioka, Satoshi, Hashimoto, Nagisa, Miyagi, Yayoi, Fukada, So-ichir, Yamamoto, Hiroshi, Tsujikawa, Kazutake
    PLoS ONE, 2014-01-21;9(1):e86367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  45. Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis.
    Authors: van Nieuwenhuijze A, Coghill E, Gray D, Prato S, Metcalf D, Alexander W, Wicks I
    Am J Pathol, 2013-10-30;184(1):184-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  46. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
    Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
    Mucosal Immunol, 2012-09-12;6(2):393-404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  47. HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4.
    Authors: Schiraldi M, Raucci A, Munoz LM, Livoti E, Celona B, Venereau E, Apuzzo T, De Marchis F, Pedotti M, Bachi A, Thelen M, Varani L, Mellado M, Proudfoot A, Bianchi ME, Uguccioni M
    J. Exp. Med., 2012-02-27;209(3):551-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  48. Calcitonin gene-related peptide is an important regulator of cutaneous immunity: effect on dendritic cell and T cell functions.
    J. Immunol., 2011-05-06;186(12):6886-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. Antibiotic-Induced Dysbiosis of Gut Microbiota Impairs Corneal Nerve Regeneration by Affecting CCR2-Negative Macrophage Distribution
    Authors: Jun Liu, Mingjuan Wu, Jingxin He, Chengju Xiao, Yunxia Xue, Ting Fu et al.
    The American Journal of Pathology
  50. Loss of Fractalkine Signaling Exacerbates Axon Transport Dysfunction in a Chronic Model of Glaucoma
    Authors: Kevin T. Breen, Sarah R. Anderson, Michael R. Steele, David J. Calkins, Alejandra Bosco, Monica L. Vetter
    Frontiers in Neuroscience
  51. LKB1 loss promotes endometrial cancer progression via CCL2-dependent macrophage recruitment.
    Authors: Pena CG, Nakada Y, Saatcioglu HD et al.
    J Clin Invest
  52. Organism-Level Analysis of Vaccination Reveals Networks of Protection across Tissues
    Authors: Motohiko Kadoki, Ashwini Patil, Cornelius C. Thaiss, Donald J. Brooks, Surya Pandey, Deeksha Deep et al.
    Cell
  53. CXCL10 chemokine regulates heterogeneity of the CD8+ T cell response and viral set point during chronic infection
    Authors: Aleksandra J. Ozga, Melvyn T. Chow, Mateus E. Lopes, Rachel L. Servis, Mauro Di Pilato, Philippe Dehio et al.
    Immunity
  54. Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution
    Authors: Mingjuan Wu, Jun Liu, Fanying Li, Shuoya Huang, Jingxin He, Yunxia Xue et al.
    Mucosal Immunology

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Mouse CCR2 APC-conjugated Antibody
By Jim Hsiao on 05/23/2017
Application: Flow Sample Tested: Lung tissue Species: Mouse

Single cell suspensions of mouse cells were isolated from the lung tissue and were blocked with Fc block for 30 minutes. Cells were then stained with the APC-conjugated anti-mouse CCR2 antibody (1: 100 in FACS buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.
Data was pregated with FSC/SSC --> FSC-A/FSC-W


Mouse CCR2 APC-conjugated Antibody
By Kihong Lim on 09/29/2016
Application: Flow Sample Tested: activated mouse CD8 T cell Species: Mouse

CCR2 expression on mouse CD8 T cell in vitro activated or virus-infected lung


Mouse CCR2 APC-conjugated Antibody
By Anonymous on 08/24/2016
Application: Flow Sample Tested: Tumor cell lyastes Species: Mouse