Mouse CCR3 Antibody

Catalog # Availability Size / Price Qty
MAB729-100
MAB729-SP
Detection of CCR3 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
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Mouse CCR3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR3 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 83101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and (A) Rat Anti-Mouse CCR3 Monoclonal Antibody (Catalog # MAB729) or (B) Rat IgG2A Isotype Control Antibody (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Mouse CCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis
    Authors: HN Um, JO Baek, S Park, EH Lee, J Jang, WJ Park, JY Roh, Y Jung
    Cell Death & Disease, 2021-03-04;12(3):243.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Eosinophils support adipocyte maturation and promote glucose tolerance in obesity
    Authors: EH Lee, M Itan, J Jang, HJ Gu, P Rozenberg, MK Mingler, T Wen, J Yoon, SY Park, JY Roh, CS Choi, WJ Park, A Munitz, Y Jung
    Sci Rep, 2018-07-02;8(1):9894.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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