Mouse CD161/NK1.1 Antibody

Catalog #: MAB7614 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7614
MAB7614-SP

Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry.

1 Image

All CD161/NK1.1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD161/NK1.1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD161/NK1.1 expressed specifically in the C57/B6 mouse strain. No detection of CD161/NK1.1 was observed in the BALB/c mouse strain. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) KLRB-1B is observed.

Source

Monoclonal Rat IgG_2A Clone # 694370

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD161/NK1.1
Val62-Ser220
Accession # P27814

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse CD161/NK1.1 Monoclonal Antibody (Catalog # MAB7614) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD161/NK1.1

KLRB1C, also known as NK1.1, CD161c, Ly-55, and NKR-P1c, is a 28 kDa type 2 transmembrane protein in the killer cell lectin-like receptor family. CD161 is expressed as a disulfide-linked homodimer on the surface of NK cells and subpopulations of NKT, CD4⁺, CD8⁺, and gamma /δ T cells. Its cross-linking on NK cells induces cytolytic activity, and on CD4 cells it promotes IgE production and the expansion of Th2 responses. Mature mouse CD161 consists of a 45 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 157 aa extracellular domain with one C-type lectin domain.

Entrez Gene IDs

17059 (Mouse)

Alternate Names

CD161 antigen; CD161; CD161/NK1.1; CD161c; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; Klrb1c; Ly55c; Ly-59; Natural killer cell surface protein P1A; NKR-P1; NKR-P1AMGC138614; NKRP1ANKR