Mouse CD3 APC-conjugated Antibody

Name: Mouse CD3 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB4841A
Rating: 5 (1 reviews)

Catalog #: FAB4841A 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4841A-100
FAB4841A-025

Detection of CD3 in Mouse Splenocytes by Flow Cytometry.

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Mouse CD3 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.

Source

Monoclonal Rat IgG_2B Clone # 17A2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

T cell hybridoma D1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) or (B) Rat IgG_2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

References

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE