Mouse CD3 PerCP-conjugated Antibody

Catalog #: FAB4841C
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4841C-100
FAB4841C-025

Detection of CD3 in Mouse Splenocytes by Flow Cytometry.

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Citations (2)

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Mouse CD3 PerCP-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.

Source

Monoclonal Rat IgG_2B Clone # 17A2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

T cell hybridoma D1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB4841C) or (B) Rat IgG_2BPerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

References

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse CD3 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Systemic administration of ?-glucan of 200ï¿½kDa modulates melanoma microenvironment and suppresses metastatic cancer
Authors: M Zhang, L Chun, V Sandoval, H Graor, J Myers, J Nthale, P Rauhe, Z Senders, K Choong, AY Huang, J Kim
Oncoimmunology, 2017-10-30;7(2):e1387347.
Species: Mouse
Sample Types: Whole Cells
Applications: FACS
Harnessing oncolytic virus-mediated antitumor immunity in an infected cell vaccine.
Authors: Lemay C, Rintoul J, Kus A, Paterson J, Garcia V, Falls T, Ferreira L, Bridle B, Conrad D, Tang V, Diallo J, Arulanandam R, Le Boeuf F, Garson K, Vanderhyden B, Stojdl D, Lichty B, Atkins H, Parato K, Bell J, Auer R
Mol Ther, 2012-07-03;20(9):1791-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry