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Mouse CD36/SR-B3 Antibody

Catalog #: AF2519
30 Citations Datasheet / COA / SDS
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AF2519
AF2519-SP
Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry.
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Citations (30)
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Mouse CD36/SR-B3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
Gly30-Lys439
Accession # Q3UAI3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD36/SR-B3 Fc Chimera (Catalog # 2519-CD)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Mouse CD36/SR-B3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CD36/SR‑B3 Biotinylated Antibody (Catalog # BAF2519)

Standard: Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF (Catalog # 2519-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD36/SR-B3 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry CD36/SR-B3 antibody in Mouse Kidney by Immunohistochemistry (IHC-P). View Larger

CD36/SR‑B3 in Mouse Kidney. CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

References
  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
  2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
  3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
  4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
  5. Malaud, E. et al. (2002) Biochem. J. 364:507.
  6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
  7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
  8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.
Long Name
Scavenger Receptor Class B, Member 3
Entrez Gene IDs
948 (Human); 12491 (Mouse)
Alternate Names
BDPLT10; CD_antigen: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; CHDS7; cluster determinant 36; Collagen R; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIb; GPIV; PAS IV; PAS-4 protein; PAS-4; PASIV; Platelet collagen receptor; platelet glycoprotein 4; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

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Citations for Mouse CD36/SR-B3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load
    Authors: J Sun, Y Su, J Chen, D Qin, Y Xu, H Chu, T Lu, J Dong, L Qin, W Li
    Biomedicines, 2023-02-28;11(3):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Ganglioside GM3 prevents high fat diet-induced hepatosteatosis via attenuated insulin signaling pathway
    Authors: O Tajima, Y Fujita, Y Ohmi, K Furukawa, K Furukawa
    PLoS ONE, 2023-02-24;18(2):e0281414.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Novel fat taste receptor agonists curtail progressive weight gain in obese male mice
    Authors: AS Khan, A Hichami, B Murtaza, ML Louillat-H, C Ramseyer, M Azadi, S Yesylevsky, F Mangin, F Lirussi, J Leemput, JF Merlin, A Schmitt, M Suliman, J Bayardon, S Semnanian, S Jugé, NA Khan
    Cellular and Molecular Gastroenterology and Hepatology, 2022-11-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Semaglutide May Alleviate Hepatic Steatosis in T2DM Combined with NFALD Mice via miR-5120/ABHD6
    Authors: R Li, Z Ye, D She, P Fang, G Zong, K Hu, D Kong, W Xu, L Li, Y Zhou, K Zhang, Y Xue
    Drug Design, Development and Therapy, 2022-10-12;16(0):3557-3572.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  5. Sea-Buckthorn Seed Oil Induces Proliferation of both Normal and Dysplastic Keratinocytes in Basal Conditions and under UVA Irradiation
    Authors: M Dudau, AC Vilceanu, E Codrici, S Mihai, ID Popescu, L Albulescu, I Tarcomnicu, G Moise, LC Ceafalan, ME Hinescu, AM Enciu, C Tanase
    Journal of personalized medicine, 2021-04-07;11(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. CD36 and LC3B initiated autophagy in B cells regulates the humoral immune response
    Authors: C He, S Wang, C Zhou, M He, J Wang, M Ladds, D Lianoudaki, SK Sedimbi, DP Lane, LS Westerberg, S Li, MCI Karlsson
    Autophagy, 2021-02-16;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  7. Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring
    Authors: RS Gaspar, AJ Unsworth, A Al-Dibouni, AP Bye, T Sage, M Stewart, S Wells, RD Cox, JM Gibbins, D Sellayah, C E Hughes
    Scientific Reports, 2021-01-14;11(1):1473.
    Species: Mouse
    Sample Types: Plasma
    Applications: Flow Cytometry
  8. Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages
    Authors: M Koga, Y Kanaoka, K Inada, S Omine, Y Kataoka, A Yamauchi
    J. Pharmacol. Sci., 2020-02-28;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  9. IL-34 promotes foam cell formation by enhancing CD36 expression through p38 MAPK pathway
    Authors: Q Liu, J Fan, J Bai, L Peng, T Zhang, L Deng, G Wang, Y Zhao, J Nong, M Zhang, Y Wang
    Sci Rep, 2018-11-26;8(1):17347.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  10. Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice
    Authors: L Håversen, JP Sundelin, A Mardinoglu, M Rutberg, M Ståhlman, U Wilhelmsso, LM Hultén, M Pekny, P Fogelstran, JF Bentzon, M Levin, J Borén
    Sci Rep, 2018-11-19;8(1):16973.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  11. Metabolomic insights of macrophage responses to graphene nanoplatelets: Role of scavenger receptor CD36
    Authors: SX Adamson, R Wang, W Wu, B Cooper, J Shannahan
    PLoS ONE, 2018-11-07;13(11):e0207042.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Inhibition of CD36 reduces visceral fat accumulation and improves insulin resistance in diet-induced obese BDNF Val66Met mice
    Authors: J Yang, KW Park, S Cho
    J. Biol. Chem., 2018-06-18;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae
    Authors: S Tsuzuki, S Lee, Y Kimoto, T Sugawara, Y Manabe, K Inoue
    Biomed. Res., 2018-01-01;39(6):295-300.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity
    Authors: S Tsuzuki, Y Kimoto, S Lee, T Sugawara, Y Manabe, K Inoue
    Biomed. Res., 2018-01-01;39(3):117-129.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Partitioning of adipose lipid metabolism by altered expression and function of PPAR isoforms after bariatric surgery
    Authors: C Jahansouz, H Xu, AV Hertzel, S Kizy, KA Steen, R Foncea, FJ Serrot, N Kvalheim, G Luthra, K Ewing, DB Leslie, S Ikramuddin, DA Bernlohr
    Int J Obes (Lond), 2017-08-14;42(2):139-146.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  16. CCN3 Regulates Macrophage Foam Cell Formation and Atherosclerosis
    Authors: H Shi, C Zhang, V Pasupuleti, X Hu, DA Prosdocimo, W Wu, Y Qing, S Wu, H Mohammad, SL Gerson, B Perbal, PA Klenotic, N Dong, Z Lin
    Am. J. Pathol., 2017-06-01;187(6):1230-1237.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. CD36 is essential for endurance improvement, changes in�whole-body metabolism, and efficient PPAR-related transcriptional responses in the muscle with exercise training
    Authors: MCC Manio, S Matsumura, D Masuda, K Inoue
    Physiol Rep, 2017-05-01;5(10):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. 2Intestinal epithelial cell Caveolin 1 regulates fatty acid and lipoprotein cholesterol plasma levels
    Authors: JP Otis, MC Shen, V Quinlivan, JL Anderson, SA Farber
    Dis Model Mech, 2017-01-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Cell-surface CD36 in monocyte/macrophage contributes to phagocytosis during the resolution phase of ischemic stroke in mice
    J Biol Chem, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide
    Authors: R Biedro?, A Peru?, S Józefowski
    PLoS ONE, 2016-04-13;11(4):e0153558.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  21. Expression of CD36 by Olfactory Receptor Cells and Its Abundance on the Epithelial Surface in Mice.
    Authors: Lee S, Eguchi A, Tsuzuki S, Matsumura S, Inoue K, Iwanaga T, Masuda D, Yamashita S, Fushiki T
    PLoS ONE, 2015-07-17;10(7):e0133412.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. Regulation of AMPK activation by CD36 links fatty acid uptake to beta-oxidation.
    Authors: Samovski D, Sun J, Pietka T, Gross R, Eckel R, Su X, Stahl P, Abumrad N
    Diabetes, 2014-08-25;64(2):353-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting.
    Authors: Pietka, Terri A, Sulkin, Matthew, Kuda, Ondrej, Wang, Wei, Zhou, Dequan, Yamada, Kathryn, Yang, Kui, Su, Xiong, Gross, Richard, Nerbonne, Jeanne M, Efimov, Igor R, Abumrad, Nada A
    J Biol Chem, 2012-09-27;287(46):38901-12.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice.
    Authors: Nagashima M, Watanabe T, Terasaki M
    Diabetologia, 2011-07-24;54(10):2649-59.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors.
    Authors: Makinen PI, Lappalainen JP, Heinonen SE, Leppanen P, Lahteenvuo MT, Aarnio JV, Heikkila J, Turunen MP, Yla-Herttuala S
    Cardiovasc. Res., 2010-07-15;88(3):530-8.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  26. Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity.
    Authors: Pi J, Leung L, Xue P, Wang W, Hou Y, Liu D, Yehuda-Shnaidman E, Lee C, Lau J, Kurtz TW, Chan JY
    J. Biol. Chem., 2010-01-20;285(12):9292-300.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
    Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
    Atherosclerosis, 2008-11-17;204(2):405-11.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury.
    Authors: Kim E, Tolhurst AT, Qin LY, Chen XY, Febbraio M, Cho S
    J. Neurosci., 2008-04-30;28(18):4661-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Transcriptional regulation of fatty acid translocase/CD36 expression by CCAAT/enhancer-binding protein alpha.
    Authors: Qiao L, Zou C, Shao P, Schaack J, Johnson PF, Shao J
    J. Biol. Chem., 2008-02-08;283(14):8788-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
    Authors: Chang Y, Finnemann SC
    J. Cell. Sci., 2007-08-07;120(0):3053-63.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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