Mouse CD45 Antibody Summary
Gln24-Lys425
Accession # NP_035340
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
CD45 in RAW 264.7 Mouse Cell Line. CD45 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD45 in Mouse Liver. CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD45
Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.
Product Datasheets
Citations for Mouse CD45 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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mTORC1 hyperactivation and resultant suppression of macroautophagy contribute to the induction of cardiomyocyte necroptosis by catecholamine surges
Authors: Cai, M;Wu, P;Ni, W;Huang, D;Wang, X;
Physiological reports
Species: S. agalactiae
Sample Types: Cell Lysates
Applications: Western Blot -
Fibroblasts facilitate lymphatic vessel formation in transplanted heart
Authors: Gong, H;Wang, T;Sun, X;Zhang, Y;Qiu, Y;Sun, W;Zhang, Y;Teng, P;Hu, Y;Hu, X;Ma, L;Xu, Q;Zhao, H;
Theranostics
Species: Murine polyomavirus strain A3, Viral
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo
Authors: Gonzalez Galofre, ZN;Kilpatrick, AM;Marques, M;Sá da Bandeira, D;Ventura, T;Gomez Salazar, M;Bouilleau, L;Marc, Y;Barbosa, AB;Rossi, F;Beltran, M;van de Werken, HJG;van IJcken, WFJ;Henderson, NC;Forbes, SJ;Crisan, M;
Nature communications
Species: Murine polyomavirus strain A3
Sample Types: Embryo
Applications: Immunohistochemistry -
Innate and adaptive immune cell interaction drives inflammasome activation and hepatocyte apoptosis in murine liver injury from immune checkpoint inhibitors
Authors: Shojaie, L;Bogdanov, JM;Alavifard, H;Mohamed, MG;Baktash, A;Ali, M;Mahov, S;Murray, S;Kanel, GC;Liu, ZX;Ito, F;In, GK;Merchant, A;Stohl, W;Dara, L;
Cell death & disease
Species: Viral
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Focused Ultrasound Blood-Brain Barrier Opening Arrests the Growth and Formation of Cerebral Cavernous Malformations
Authors: Fisher, DG;Sharifi, KA;Shah, IM;Gorick, CM;Breza, VR;Debski, AC;Hoch, MR;Cruz, T;Samuels, JD;Sheehan, JP;Schlesinger, D;Moore, D;Lukens, JR;Miller, GW;Tvrdik, P;Price, RJ;
bioRxiv : the preprint server for biology
Species: Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Antibody-Mediated Delivery of VEGF-C Promotes Long-Lasting Lymphatic Expansion That Reduces Recurrent Inflammation
Authors: N Cousin, S Bartel, J Scholl, C Tacconi, A Egger, G Thorhallsd, D Neri, LC Dieterich, M Detmar
Cells, 2022-12-31;12(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss
Authors: A Xia, A Thai, Z Cao, X Chen, J Chen, B Bacacao, LA Bekale, V Schiel, PL Bollyky, PLS Maria
Journal of Neuroinflammation, 2022-09-12;19(1):224.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice
Authors: K Xu, S Chen, L Xie, Y Qiu, XZ Liu, X Bai, Y Jin, XH Wang, Y Sun
Cell Death & Disease, 2022-06-10;13(6):545.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition
Authors: G Huang, Z Cheng, A Hildebrand, C Wang, M Cimini, R Roy, AM Lucchese, C Benedict, V Mallaredy, A Magadum, D Joladarash, C Thej, C Gonzalez, M Trungcao, VNS Garikipati, JW Elrod, WJ Koch, R Kishore
Theranostics, 2022-05-21;12(9):4415-4430.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dose and Dose Rate-Dependent Effects of Low-Dose Irradiation on Inflammatory Parameters in ApoE-Deficient and Wild Type Mice
Authors: A Glasow, I Patties, ND Priest, REJ Mitchel, G Hildebrand, K Manda
Cells, 2021-11-20;10(11):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
Authors: J Wang, A Rattner, J Nathans
Elife, 2021-11-16;10(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection
Authors: J Filtjens, A Roger, L Quatrini, E Wieduwild, J Gouilly, G Hoeffel, R Rossignol, C Daher, G Debroas, S Henri, CM Jones, B Malissen, LK Mackay, A Moqrich, FR Carbone, S Ugolini
Nature Communications, 2021-05-18;12(1):2936.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Intertumoral Genetic Heterogeneity Generates Distinct Tumor Microenvironments in a Novel Murine Synchronous Melanoma Model
Authors: SS Qin, BJ Han, A Williams, KM Jackson, R Jewell, AC Chacon, EM Lord, DC Linehan, M Kim, A Reuben, SA Gerber, PA Prieto
Cancers, 2021-05-11;13(10):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade
Authors: RM Lasagni Vi, M Barbariga, P Fonteyne, F Bignami, P Rama, G Ferrari
Investigative Ophthalmology & Visual Science, 2021-03-01;62(3):26.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Airway-Associated Macrophages in Homeostasis and Repair
Authors: AE Engler, AB Ysasi, RMF Pihl, C Villacorta, HM Heston, HMK Richardson, BR Thapa, NR Moniz, AC Belkina, SA Mazzilli, JR Rock
Cell Reports, 2020-12-29;33(13):108553.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa
Authors: MT Massengill, NF Ash, BM Young, CJ Ildefonso, AS Lewin
Sci Rep, 2020-10-12;10(1):16967.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells
Authors: V Rai, MB Wood, H Feng, NM Schabla, S Tu, J Zuo
Scientific Reports, 2020-09-16;10(1):15167.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
Exp. Mol. Med., 2020-09-14;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Keratinocyte-Expressed Podoplanin is Dispensable for Multi-Step Skin Carcinogenesis
Authors: M Sesarti?, K Ikenberg, SY Yoon, M Detmar
Cells, 2020-06-24;9(6):.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice
Authors: A Shahapal, EB Cho, HJ Yong, I Jeong, H Kwak, JK Lee, W Kim, B Kim, HC Park, WS Lee, H Kim, JI Hwang, JY Seong
Front Neurosci, 2019-08-30;13(0):917.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Antibody-mediated delivery of VEGF-C potently reduces chronic skin inflammation
Authors: S Schwager, S Renner, T Hemmerle, S Karaman, ST Proulx, R Fetz, AM Golding-Oc, P Probst, C Halin, D Neri, M Detmar
JCI Insight, 2018-12-06;3(23):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
STAT5A/B BLOCKADE SENSITIZES PROSTATE CANCER TO RADIATION THROUGH INHIBITION OF RAD51 AND DNA REPAIR
Authors: C Maranto, V Udhane, DT Hoang, L Gu, V Alexeev, KM Malas, K Cardenas, JR Brody, U Rodeck, C Bergom, KA Iczkowski, K Jacobsohn, WA See, SM Schmitt, MT Nevalainen
Clin. Cancer Res., 2018-02-26;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Type I interferons instigate fetal demise after Zika virus infection
Authors: LJ Yockey, KA Jurado, N Arora, A Millet, T Rakib, KM Milano, AK Hastings, E Fikrig, Y Kong, TL Horvath, S Weatherbee, HJ Kliman, CB Coyne, A Iwasaki
Sci Immunol, 2018-01-05;3(19):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
JCI Insight, 2017-09-21;2(18):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss
Authors: MX Hernandez, S Jiang, TA Cole, SH Chu, MI Fonseca, MJ Fang, LA Hohsfield, MD Torres, KN Green, RA Wetsel, A Mortazavi, AJ Tenner
Mol Neurodegener, 2017-09-18;12(1):66.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Metabolic origins of spatial organization in the tumor microenvironment
Authors: C Carmona-Fo, M Deforet, L Akkari, CB Thompson, JA Joyce, JB Xavier
Proc. Natl. Acad. Sci. U.S.A, 2017-02-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain
Authors: Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao
Neurobiol. Dis, 2017-02-09;101(0):27-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
Authors: Jong-Gil Park
Sci Rep, 2016-06-15;6(0):27938.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
PLoS ONE, 2015-03-23;10(3):e0119661.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
Authors: Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A
Development, 2014-09-01;141(17):3319-23.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia.
J Neuroinflammation, 2012-07-16;9(0):174.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine.
Authors: Gobbetti T, Cenac N, Motta JP, Rolland C, Martin L, Andrade-Gordon P, Steinhoff M, Barocelli E, Vergnolle N
Am. J. Pathol., 2011-11-07;180(1):141-52.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP
J. Biol. Chem., 2011-01-24;286(13):10918-29.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
Science, 2010-11-05;330(6005):827-30.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
Authors: Graham BB, Mentink-Kane MM, El-Haddad H
Am. J. Pathol., 2010-07-29;177(3):1549-61.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
Authors: Chou S, Lodish HF
Proc. Natl. Acad. Sci. U.S.A., 2010-04-12;107(17):7799-804.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages.
Authors: Tigges U, Hyer EG, Scharf J, Stallcup WB
Development, 2008-01-02;135(3):523-32.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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