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Mouse CD45 Antibody

Catalog #: AF114
37 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF114
AF114-SP
CD45 in RAW 264.7 Mouse Cell Line.
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Product Details
Citations (37)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD45 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD45 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD45
Gln24-Lys425
Accession # NP_035340
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD45 (Catalog # 114-CD)
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

CD45 in RAW 264.7 Mouse Cell Line. CD45 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD45 antibody in Mouse Liver by Immunohistochemistry (IHC-P). View Larger

CD45 in Mouse Liver. CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45

Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.

Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citations for Mouse CD45 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. mTORC1 hyperactivation and resultant suppression of macroautophagy contribute to the induction of cardiomyocyte necroptosis by catecholamine surges
    Authors: Cai, M;Wu, P;Ni, W;Huang, D;Wang, X;
    Physiological reports
    Species: S. agalactiae
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Fibroblasts facilitate lymphatic vessel formation in transplanted heart
    Authors: Gong, H;Wang, T;Sun, X;Zhang, Y;Qiu, Y;Sun, W;Zhang, Y;Teng, P;Hu, Y;Hu, X;Ma, L;Xu, Q;Zhao, H;
    Theranostics
    Species: Murine polyomavirus strain A3, Viral
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo
    Authors: Gonzalez Galofre, ZN;Kilpatrick, AM;Marques, M;Sá da Bandeira, D;Ventura, T;Gomez Salazar, M;Bouilleau, L;Marc, Y;Barbosa, AB;Rossi, F;Beltran, M;van de Werken, HJG;van IJcken, WFJ;Henderson, NC;Forbes, SJ;Crisan, M;
    Nature communications
    Species: Murine polyomavirus strain A3
    Sample Types: Embryo
    Applications: Immunohistochemistry
  4. Innate and adaptive immune cell interaction drives inflammasome activation and hepatocyte apoptosis in murine liver injury from immune checkpoint inhibitors
    Authors: Shojaie, L;Bogdanov, JM;Alavifard, H;Mohamed, MG;Baktash, A;Ali, M;Mahov, S;Murray, S;Kanel, GC;Liu, ZX;Ito, F;In, GK;Merchant, A;Stohl, W;Dara, L;
    Cell death & disease
    Species: Viral
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  5. Focused Ultrasound Blood-Brain Barrier Opening Arrests the Growth and Formation of Cerebral Cavernous Malformations
    Authors: Fisher, DG;Sharifi, KA;Shah, IM;Gorick, CM;Breza, VR;Debski, AC;Hoch, MR;Cruz, T;Samuels, JD;Sheehan, JP;Schlesinger, D;Moore, D;Lukens, JR;Miller, GW;Tvrdik, P;Price, RJ;
    bioRxiv : the preprint server for biology
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. Antibody-Mediated Delivery of VEGF-C Promotes Long-Lasting Lymphatic Expansion That Reduces Recurrent Inflammation
    Authors: N Cousin, S Bartel, J Scholl, C Tacconi, A Egger, G Thorhallsd, D Neri, LC Dieterich, M Detmar
    Cells, 2022-12-31;12(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss
    Authors: A Xia, A Thai, Z Cao, X Chen, J Chen, B Bacacao, LA Bekale, V Schiel, PL Bollyky, PLS Maria
    Journal of Neuroinflammation, 2022-09-12;19(1):224.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice
    Authors: K Xu, S Chen, L Xie, Y Qiu, XZ Liu, X Bai, Y Jin, XH Wang, Y Sun
    Cell Death & Disease, 2022-06-10;13(6):545.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition
    Authors: G Huang, Z Cheng, A Hildebrand, C Wang, M Cimini, R Roy, AM Lucchese, C Benedict, V Mallaredy, A Magadum, D Joladarash, C Thej, C Gonzalez, M Trungcao, VNS Garikipati, JW Elrod, WJ Koch, R Kishore
    Theranostics, 2022-05-21;12(9):4415-4430.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Dose and Dose Rate-Dependent Effects of Low-Dose Irradiation on Inflammatory Parameters in ApoE-Deficient and Wild Type Mice
    Authors: A Glasow, I Patties, ND Priest, REJ Mitchel, G Hildebrand, K Manda
    Cells, 2021-11-20;10(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
    Authors: J Wang, A Rattner, J Nathans
    Elife, 2021-11-16;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection
    Authors: J Filtjens, A Roger, L Quatrini, E Wieduwild, J Gouilly, G Hoeffel, R Rossignol, C Daher, G Debroas, S Henri, CM Jones, B Malissen, LK Mackay, A Moqrich, FR Carbone, S Ugolini
    Nature Communications, 2021-05-18;12(1):2936.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Intertumoral Genetic Heterogeneity Generates Distinct Tumor Microenvironments in a Novel Murine Synchronous Melanoma Model
    Authors: SS Qin, BJ Han, A Williams, KM Jackson, R Jewell, AC Chacon, EM Lord, DC Linehan, M Kim, A Reuben, SA Gerber, PA Prieto
    Cancers, 2021-05-11;13(10):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade
    Authors: RM Lasagni Vi, M Barbariga, P Fonteyne, F Bignami, P Rama, G Ferrari
    Investigative Ophthalmology & Visual Science, 2021-03-01;62(3):26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Airway-Associated Macrophages in Homeostasis and Repair
    Authors: AE Engler, AB Ysasi, RMF Pihl, C Villacorta, HM Heston, HMK Richardson, BR Thapa, NR Moniz, AC Belkina, SA Mazzilli, JR Rock
    Cell Reports, 2020-12-29;33(13):108553.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa
    Authors: MT Massengill, NF Ash, BM Young, CJ Ildefonso, AS Lewin
    Sci Rep, 2020-10-12;10(1):16967.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells
    Authors: V Rai, MB Wood, H Feng, NM Schabla, S Tu, J Zuo
    Scientific Reports, 2020-09-16;10(1):15167.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
    Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
    Exp. Mol. Med., 2020-09-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Keratinocyte-Expressed Podoplanin is Dispensable for Multi-Step Skin Carcinogenesis
    Authors: M Sesarti?, K Ikenberg, SY Yoon, M Detmar
    Cells, 2020-06-24;9(6):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice
    Authors: A Shahapal, EB Cho, HJ Yong, I Jeong, H Kwak, JK Lee, W Kim, B Kim, HC Park, WS Lee, H Kim, JI Hwang, JY Seong
    Front Neurosci, 2019-08-30;13(0):917.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Antibody-mediated delivery of VEGF-C potently reduces chronic skin inflammation
    Authors: S Schwager, S Renner, T Hemmerle, S Karaman, ST Proulx, R Fetz, AM Golding-Oc, P Probst, C Halin, D Neri, M Detmar
    JCI Insight, 2018-12-06;3(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. STAT5A/B BLOCKADE SENSITIZES PROSTATE CANCER TO RADIATION THROUGH INHIBITION OF RAD51 AND DNA REPAIR
    Authors: C Maranto, V Udhane, DT Hoang, L Gu, V Alexeev, KM Malas, K Cardenas, JR Brody, U Rodeck, C Bergom, KA Iczkowski, K Jacobsohn, WA See, SM Schmitt, MT Nevalainen
    Clin. Cancer Res., 2018-02-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Type I interferons instigate fetal demise after Zika virus infection
    Authors: LJ Yockey, KA Jurado, N Arora, A Millet, T Rakib, KM Milano, AK Hastings, E Fikrig, Y Kong, TL Horvath, S Weatherbee, HJ Kliman, CB Coyne, A Iwasaki
    Sci Immunol, 2018-01-05;3(19):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
    Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
    JCI Insight, 2017-09-21;2(18):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss
    Authors: MX Hernandez, S Jiang, TA Cole, SH Chu, MI Fonseca, MJ Fang, LA Hohsfield, MD Torres, KN Green, RA Wetsel, A Mortazavi, AJ Tenner
    Mol Neurodegener, 2017-09-18;12(1):66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Metabolic origins of spatial organization in the tumor microenvironment
    Authors: C Carmona-Fo, M Deforet, L Akkari, CB Thompson, JA Joyce, JB Xavier
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain
    Authors: Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao
    Neurobiol. Dis, 2017-02-09;101(0):27-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
    Authors: Jong-Gil Park
    Sci Rep, 2016-06-15;6(0):27938.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
    Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
    PLoS ONE, 2015-03-23;10(3):e0119661.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
    Authors: Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A
    Development, 2014-09-01;141(17):3319-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia.
    J Neuroinflammation, 2012-07-16;9(0):174.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  32. Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine.
    Authors: Gobbetti T, Cenac N, Motta JP, Rolland C, Martin L, Andrade-Gordon P, Steinhoff M, Barocelli E, Vergnolle N
    Am. J. Pathol., 2011-11-07;180(1):141-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
    Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP
    J. Biol. Chem., 2011-01-24;286(13):10918-29.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
    Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
    Science, 2010-11-05;330(6005):827-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
    Authors: Graham BB, Mentink-Kane MM, El-Haddad H
    Am. J. Pathol., 2010-07-29;177(3):1549-61.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
    Authors: Chou S, Lodish HF
    Proc. Natl. Acad. Sci. U.S.A., 2010-04-12;107(17):7799-804.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  37. FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages.
    Authors: Tigges U, Hyer EG, Scharf J, Stallcup WB
    Development, 2008-01-02;135(3):523-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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