Mouse CD5 APC-conjugated Antibody

Catalog #: FAB115A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB115A-025

Detection of CD5 in Mouse Splenocytes by Flow Cytometry.

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Mouse CD5 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD5 in flow cytometry.

Source

Monoclonal Rat IgG_2A Clone # 53-7.3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse thymus or spleen

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD5 APC-conjugated Monoclonal Antibody (Catalog # FAB115A) or (B) Rat IgG_2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

References