Mouse CD59b Antibody

Catalog # Availability Size / Price Qty
AF2650
AF2650-SP
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Mouse CD59b Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD59b in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD59b
Leu24-Asn104
Accession # P58019
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD59b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD59b

CD59, also known as membrane attack complex inhibition factor (MACIF), is a GPI-linked complement regulatory protein that prevents the formation of membrane attack complex and inhibits complement mediated lysis. In mouse, the gene encoding CD59 has been duplicated, giving rise to CD59a and CD59b. The two proteins are differentially expressed in tissues and share approximately 65% amino acid sequence identity.

Entrez Gene IDs
333883 (Mouse)
Alternate Names
16.3A5;1F5;CD59 glycoprotein;EJ16;EJ30;EL32;G344;HRF20;HRF-20;MACIF;MAC-IP;MEM43;MIC11;MIN1;MIN2;MIN3;MIRL;MSK21;p18-20; CD59b

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