Mouse CD63 Antibody

Name: Mouse CD63 Antibody
Brand: R&D Systems
SKU: MAB5417
Rating: 5 (2 reviews)

Catalog #: MAB5417
3 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5417
MAB5417-SP

Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry.

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All CD63 Antibodies

Product Details

Citations (3)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD63 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD63 in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 446703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse CD63
Met1-Met238
Accession # P41731

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CD63 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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CD63 in Mouse Kidney. CD63 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.

Entrez Gene IDs

967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)

Alternate Names

CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CD63 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells
Authors: JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng
Nature Cell Biology, 2021-12-30;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS
Authors: Y Men, J Yelick, S Jin, Y Tian, MSR Chiang, H Higashimor, E Brown, R Jarvis, Y Yang
Nat Commun, 2019-09-12;10(1):4136.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Bacterial colonization of host cells in the absence of cholesterol.
Authors: Gilk S, Cockrell D, Luterbach C, Hansen B, Knodler L, Ibarra J, Steele-Mortimer O, Heinzen R
PLoS Pathog, 2013-01-24;9(1):e1003107.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC