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Mouse CDCP1 Antibody

Catalog #: AF4515
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4515
AF4515-SP
CDCP1 in Mouse Embryo.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CDCP1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CDCP1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
Arg25-Ala667
Accession # Q5U462
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CDCP1
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

CDCP1 in Mouse Embryo. CDCP1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4515) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing mouse brain. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDCP1

CDCP1 (CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa’s 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350). Over aa’s 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

Long Name
CUB Domain Containing Protein 1
Entrez Gene IDs
64866 (Human); 109332 (Mouse)
Alternate Names
CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases

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Citations for Mouse CDCP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice
    Authors: Yiding Zhao, Qing Wang, Wenhao Zhang, Qingshen Jia, Qian Gao, Ling Shuai
    STAR Protocols
  2. Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
    Authors: Mei Xu, Wenhao Zhang, Mengyang Geng, Yiding Zhao, Shengyi Sun, Qian Gao et al.
    STAR Protocols
  3. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions
    Authors: A Yachie-Kin, K Onishi, J Ostblom, MA Langley, E Posfai, J Rossant, PW Zandstra
    Mol. Syst. Biol., 2018-01-29;14(1):e7952.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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