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Mouse CLEC4F/CLECSF13 Antibody

Catalog #: MAB2784
5 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2784
MAB2784-SP
CLEC4F/CLECSF13 in Mouse Liver.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CLEC4F/CLECSF13 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.
Source
Monoclonal Rat IgG2A Clone # 370901
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
Ala65-Gly548
Accession # P70194
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CLEC4F/CLECSF13 (Catalog # 2784-CL)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CLEC4F/CLECSF13 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

CLEC4F/CLECSF13 in Mouse Liver. CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CLEC4F/CLECSF13 Monoclonal Antibody (Catalog # MAB2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

References
  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
  3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
  4. Accession # P70194.
  5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
  6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
  7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
  8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
  9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
  10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
  11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.
Long Name
C-type Lectin Domain Family 4, Member F
Entrez Gene IDs
165530 (Human); 51811 (Mouse); 114598 (Rat)
Alternate Names
CLEC4F; CLECSF13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13; C-type lectin 13; C-type lectin domain family 4, member F; Galactose Particle Receptor; KCLR; KCR

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Citations for Mouse CLEC4F/CLECSF13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Characteristic gene expression in the liver monocyte-macrophage-DC system is associated with the progression of fibrosis in NASH
    Authors: Wang X, Wang Z, Liu B et al.
    Frontiers in Immunology
  2. Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice
    Authors: Y Jiang, Y Tang, C Hoover, Y Kondo, D Huang, D Restagno, B Shao, L Gao, J Michael Mc, M Zhou, R Silasi-Man, S McGee, M Jiang, X Bai, F Lupu, C Ruan, JD Marth, D Wu, Y Han, L Xia
    Cell Death and Differentiation, 2021-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. In vivo liposomal delivery of PPARalpha/gamma dual agonist tesaglitazar in a model of obesity enriches macrophage targeting and limits liver and kidney drug effects
    Authors: V Osinski, DK Bauknight, SSK Dasa, MJ Harms, T Kroon, MA Marshall, JC Garmey, AT Nguyen, J Hartman, A Upadhye, P Srikakulap, A Zhou, G O'Mahony, AL Klibanov, KA Kelly, J Boucher, CA McNamara
    Theranostics, 2020-01-01;10(2):585-601.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Adipocyte Death Preferentially Induces Liver Injury and Inflammation Through the Activation of Chemokine (C-C Motif) Receptor 2-Positive Macrophages and Lipolysis
    Authors: Seung-Jin K, Dechun F, Adrien G et al.
    Hepatology.
  5. Lipopolysaccharide Lowers Cholesteryl Ester Transfer Protein by Activating F4/80+Clec4f+Vsig4+Ly6C- Kupffer Cell Subsets
    Authors: SJL van der Tu, Z Li, JFP Berbée, I Verkouter, LE Ringnalda, AE Neele, JB van Klinke, SS Rensen, J Fu, MPJ de Winther, AK Groen, PCN Rensen, K Willems va, Y Wang
    J Am Heart Assoc, 2018-03-10;7(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse CLEC4F/CLECSF13 Antibody

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Mouse CLEC4F/CLECSF13 Antibody
By Anonymous on 11/23/2021
Application: IHC Sample Tested: Liver tissue Species: Mouse
Immunohistochemistry Mouse CLEC4F/CLECSF13 Antibody MAB2784
Mouse CLEC4F/CLECSF13 Antibody
By Anonymous on 02/21/2018
Application: Flow Sample Tested: Liver cells Species: Mouse

Indirect staining of Kupffer cells with Goat-Anti Rat AF647 secondary antibody in mouse livers.

Flow Cytometry Mouse CLEC4F/CLECSF13 Antibody MAB2784