Mouse CLEC9a Antibody Summary
Accession # Q8BRU4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CLEC9a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67761) or (B) Rat IgG1Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC9a
CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.
Product Datasheets
Citations for Mouse CLEC9a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates.
Authors: Li J, Ahmet F, Sullivan L, Brooks A, Kent S, De Rose R, Salazar A, Reis E Sousa C, Shortman K, Lahoud M, Heath W, Caminschi I
Eur J Immunol, 2015-01-14;45(3):854-64.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas.
Authors: Picco G, Beatson R, Taylor-Papadimitriou J, Burchell J
Eur J Immunol, 2014-04-17;44(7):1947-55.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
Boosting antibody responses by targeting antigens to dendritic cells.
Authors: Caminschi I, Shortman K
Trends Immunol, 2011-12-06;33(2):71-7.
Applications: Functional Assay -
Efficient and versatile manipulation of the peripheral CD4+ T-cell compartment by antigen targeting to DNGR-1/CLEC9A.
Authors: Joffre O, Sancho D, Zelenay S, Keller A, Reis E Sousa C
Eur J Immunol, 2010-05-01;40(5):1255-65.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Capture)
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