Mouse Coagulation Factor III/Tissue Factor Antibody

Catalog # Availability Size / Price Qty
MAB3178
MAB3178-SP
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Mouse Coagulation Factor III/Tissue Factor Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2C Clone # 355220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III
Ala29-Glu251
Accession # P20352
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 - 28) and the mature chain (residues 29 - 294). As a type I membrane protein, it contains a transmembrane region (residues 252 - 274) and a cytoplasmic tail (residues 275 - 294) (3, 4). The purified recombinant mouse TF corresponds to the ectodomain (residues 29 - 251) and is potent in activating thermolysin-processed recombinant mouse Coagulation Factor VII (R&D Systems, Catalog # 3305‑SE) under the conditions described in the Activity Assay Protocol.

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
  3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
  4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
Entrez Gene IDs
2152 (Human); 14066 (Mouse)
Alternate Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

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