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Mouse Collagen II Antibody

Catalog #: AF3615
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3615
AF3615-SP
Collagen II in Mouse Chondrocytes.
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Citations (10)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Collagen II Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Collagen II in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse sternum-derived Collagen II
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Mouse Collagen II
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Collagen II antibody in Mouse Chondrocytes by Immunocytochemistry (ICC). View Larger

Collagen II in Mouse Chondrocytes. Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen II

Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.

Entrez Gene IDs
1280 (Human); 12824 (Mouse); 25412 (Rat)
Alternate Names
Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; COL11A3; COL2A1; Collagen 2; collagen alpha-1(II) chain; Collagen II; collagen II, alpha-1 polypeptide; collagen type 2; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital); collagen, type II, alpha 1; Collagen-2; MGC131516; SEDC

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Citations for Mouse Collagen II Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Inactivation of Sox9 in fibroblasts reduces cardiac fibrosis and inflammation
    Authors: Gesine M. Scharf, Katja Kilian, Julio Cordero, Yong Wang, Andrea Grund, Melanie Hofmann et al.
    JCI Insight
  2. Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development
    Authors: So EY, Sun C, Wu KQ et al.
    J. Cell. Physiol.
  3. Vav1 Regulates Mesenchymal Stem Cell Differentiation Decision Between Adipocyte and Chondrocyte via Sirt1
    Authors: Peng Qu, Lizhen Wang, Yongfen Min, Lois McKennett, Jonathan R. Keller, P. Charles Lin
    Stem Cells
  4. SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone
    Authors: J Li, J Xue, Y Jing, M Wang, R Shu, H Xu, C Xue, J Feng, P Wang, D Bai
    Biomed Res Int, 2019-11-11;2019(0):7623562.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse
    Authors: Y Chen, H Xu, G Lin
    Cell Discov, 2017-12-19;3(0):17046.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
    Authors: Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M
    Stem Cells Int, 2014-09-10;2014(0):468927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  7. Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease.
    Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
    PLoS ONE, 2012-06-07;7(6):e38615.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
    Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
    PLoS ONE, 2012-04-12;7(4):e34703.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism.
    Authors: Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H
    J. Biol. Chem., 2010-12-10;286(7):5803-12.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase (TNAP) in Vascular Endothelium Results in Generalized Arterial Calcification
    Authors: Alexei Y. Savinov, Maryam Salehi, Manisha C. Yadav, Ilian Radichev, José Luis Millán, Olga V. Savinova
    Journal of the American Heart Association

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Mouse Collagen II Antibody
By Ying Chen on 12/14/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: embryonic leg bone Species: Mouse

mouse E17.5 embryonic leg frozen section. Primary antibody was incubated overnight at 4 °C. Secondary antibody was donkey anit-sheep 555.

Immunocytochemistry/Immunofluorescence Mouse Collagen II Antibody AF3615