Mouse Collagen II Biotinylated Antibody

Catalog #: BAF3615 Datasheet / COA / SDS

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BAF3615

All Collagen II Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Collagen II Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Collagen II in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse sternum-derived Collagen II

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Mouse Collagen II

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen II

Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.

Entrez Gene IDs

1280 (Human); 12824 (Mouse); 25412 (Rat)

Alternate Names

Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; COL11A3; COL2A1; Collagen 2; collagen alpha-1(II) chain; Collagen II; collagen II, alpha-1 polypeptide; collagen type 2; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital); collagen, type II, alpha 1; Collagen-2; MGC131516; SEDC