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Mouse Complement Component C5a Antibody

Catalog #: AF2150
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2150
AF2150-SP
N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Complement Component C5a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Complement Component C5a in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), approximately 50% cross-reactivity with recombinant human Complement Component C5a is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Complement Component C5a
Asn679-Arg755
Accession # P06684
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Complement Component C5a (Catalog # 2150-C5)
Neutralization
Measured by its ability to neutralize Complement ComponentC5a-induced N-acetyl-beta -D-glucosaminidase release in the dibutyryl cyclic-AMPdifferentiated U937 human histiocytic lymphoma cell line. Gerard, NP and Gerard, C. (1990) Biochemistry29:9274. The Neutralization Dose (ND50) istypically 0.3-1.8 μg/mL in the presence of 100 ng/mL Recombinant MouseComplement Component C5a and cytochalasin‑B.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody. View Larger

N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody. Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) inducesN-acetyl- beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl- beta -D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Complement Component C5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2150). The ND50 is typically 0.3-1.8 µg/mL in the presence of cytochalasin-B.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C5a

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a non-signaling GPCR termed C5L2 whose function is yet to be determined (5).

References
  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
  5. Okinaga, S. et al. (2003) Biochemistry 42:9406.
Entrez Gene IDs
727 (Human); 15139 (Mouse)
Alternate Names
C5; C5A; Complement Component C5a; HE

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Citations for Mouse Complement Component C5a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Complement C3 Deficiency Attenuates Chronic Hypoxia-Induced Pulmonary Hypertension in Mice
    Authors: Eileen M. Bauer, Han Zheng, Suzy Comhair, Serpil Erzurum, Timothy R. Billiar, Philip M. Bauer
    PLoS ONE
  2. Primed CD8(+) T-cell responses to allogeneic endothelial cells are controlled by local complement activation.
    Authors: Raedler H, Yang M, Lalli PN
    Am. J. Transplant., 2009-06-26;9(8):1784-95.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Decay accelerating factor can control T cell differentiation into IFN-gamma-producing effector cells via regulating local C5a-induced IL-12 production.
    Authors: Lalli PN, Strainic MG, Lin F, Medof ME, Heeger PS
    J. Immunol., 2007-11-01;179(9):5793-802.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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