Mouse Contactin-3 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Mouse Contactin-3 antibody by Western Blot. Detection of Mouse Contactin-3 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Contactin-3_MAB6644_Western_Blot_10865.jpg)
Detection of Mouse Contactin‑3 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Contactin-3 Monoclonal Antibody (Catalog # MAB6644) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Contactin-3 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
![Detection of CNTN-3 antibody in bEnd.3 mouse endothelioma cell line antibody by Flow Cytometry. Detection of CNTN-3 antibody in bEnd.3 mouse endothelioma cell line antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/Contactin-3_MAB6644_Flow_Cytometry_10668.jpg)
Detection of CNTN-3 in bEnd.3 mouse endothelioma cell line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Contactin-3 Monoclonal Antibody (Catalog # MAB6644, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
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Preparation and Storage
Background: Contactin-3
Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons, and olfactory granule cells. It likely mediates cell-cell interaction and may promote neurite outgrowth. The mouse Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region, and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG-like domains (aa 32-593), followed by four Fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, mouse Contactin-3 shares 92% and 96% aa identity with human and rat Contactin-3, respectively.
Product Datasheets
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