Mouse CXCR1/IL-8RA APC-conjugated Antibody

Catalog #: FAB8628A Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB8628A-025
FAB8628A-100

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected With Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry.

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All CXCR1/IL-8RA Antibodies

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Mouse CXCR1/IL-8RA APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCR1 transfectants but not CXCR2 transfectants in flow cytometry.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1122A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

A peptide corresponding to the N-terminal amino acid sequence of mouse CXCR1
Accession # NP_839972

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected With Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) mouse CXCR2/IL-8 RB and eGFP was stained with Rabbit Anti-Mouse CXCR1/IL-8 RA APC-conjugated Monoclonal Antibody (Catalog # FAB8628A). Quadrant markers were set based on control antibody staining (Catalog # IC105A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR1/IL-8RA

CXCR1 is a G protein-linked seven transmembrane domain spanning chemokine receptor that binds to mouse GCP-2, which is a powerful chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. The chemokine receptors CXCR1 and CXCR2 play an important role in mediating neutrophil recruitment and neutrophil-dependent injury in several models of inflammation.

Long Name

Interleukin 8 Receptor A

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Alternate Names

CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 RA; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A