Mouse CXCR7/RDC-1 Antibody

Catalog #: AF4227
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4227
AF4227-SP

Detection of Mouse CXCR7/RDC‑1 by Western Blot.

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All CXCR7/RDC-1 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR7/RDC-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects CXCR7/RDC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CXCR5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CXCR7 extracellular N-terminus and loops Met1-Leu47, Ser103-Lys118, Lys184-Glu213, and Leu274-Ala296 Accession # P56485

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CXCR7/RDC‑1 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CXCR7/RDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CXCR7/RDC-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry

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Detection of CXCR7/RDC‑1 in Mouse Neural Progenitor Cells by Flow Cytometry. Undifferentiated mouse neural progenitor cells were stained with Sheep Anti-Mouse CXCR7/RDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4227, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: CXCR7/RDC-1

CXCR7 (CXC chemokine receptor 7; also GPRN1, RDC1 and chemokine orphan receptor 1) is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF1, and appears to involve beta -arrestin2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF1, and I-TAC may actually block some SDF1-mediated migration activity. Mouse CXCR7 is a 7-transmembrane glycoprotein that is 362 amino acids (aa) in length. It contains a 47 aa N-terminal extracellular region plus a 43 aa C-terminal cytoplasmic domain. Over aa 1-47, 103-118, 184-213 and 274-296 collectively, mouse CXCR7 shares 97% and 91% aa identity with rat and human CXCR7, respectively.

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Alternate Names

ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CXCR7/RDC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
PLoS ONE, 2012-04-12;7(4):e34608.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs.
Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
Biochem. Biophys. Res. Commun., 2010-09-24;401(4):509-15.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot

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