Mouse Cyr61/CCN1 Antibody Summary
Asp176-Gly281
Accession # NP_034646
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Cyr61/CCN1 by Western Blot. Western blot shows lysates of mouse spleenocyte cell, RAW 264.7 mouse monocyte/macrophage cell line, NIH-3T3 mouse embryonic fibroblast cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cyr61/CCN1 in Mouse Embryo. Cyr61/CCN1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Mouse Cyr61/CCN1 by Simple WesternTM. Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 51 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyr61/CCN1
Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated protein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176‑281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.
Product Datasheets
Citations for Mouse Cyr61/CCN1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2
Authors: N Cheng, KH Kim, LF Lau
JCI Insight, 2022-06-16;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Amlodipine accelerates bone healing in a stable closed femoral fracture model in mice
Authors: MM Menger, B Merscher, C Scheuer, BJ Braun, SC Herath, MF Rollmann, D Stenger, T Später, T Pohlemann, MD Menger, T Histing
European cells & materials, 2021-05-24;41(0):592-602.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury
Authors: D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi
Sci Rep, 2019-11-13;9(1):16736.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation
Proc Natl Acad Sci USA, 2016-08-01;113(33):E4847-56.
Species: Mouse
Sample Types: Cell Culture Supernates, In Vivo, Whole Cells
Applications: Direct ELISA, IHC-Fr, Neutralization -
CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB.
Authors: Kim K, Chen C, Alpini G, Lau L
J Clin Invest, 2015-03-30;125(5):1886-900.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The matricellular protein Cyr61 is a key mediator of platelet-derived growth factor-induced cell migration.
Authors: Zhang F, Hao F, An D, Zeng L, Wang Y, Xu X, Cui M
J Biol Chem, 2015-01-26;290(13):8232-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Immunoprecipitation -
Matricellular protein CCN1 promotes regression of liver fibrosis through induction of cellular senescence in hepatic myofibroblasts.
Authors: Kim K, Chen C, Monzon R, Lau L
Mol Cell Biol, 2013-03-18;33(10):2078-90.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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