Mouse Cystatin C Antibody

Catalog #: MAB1238 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1238
MAB1238-SP

Detection of Mouse Cystatin C by Western Blot.

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All Cystatin C Antibodies

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Mouse Cystatin C Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse (rm) Cystatin C in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) Cystatin C is observed, but no cross-reactivity with rhCystatins A, D, S, SA or SN, rmCystatins B or EM, rhFetuin B, rmFetuin A, rhHPRG, or rhKininogen is observed.

Source

Monoclonal Rat IgG_2B Clone # 207103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C aa 21-140 Accession # P21460

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Cystatin C by Western Blot. Western blot shows lysates of mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Cystatin C Monoclonal Antibody (Catalog # MAB1238) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is an inhibitor of cysteine proteases including Cathepsins B, H, K, L, and S. It is ubiquitous in mouse tissues with highest expression levels in brain.

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Alternate Names

ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; Cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin