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Mouse DPPA4 Antibody

Catalog #: AF3730
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3730
AF3730-SP
DPPA4 in D3 Mouse Cell Line.
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Citations (12)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DPPA4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DPPA4 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DPPA4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse DPPA4
Asn125-Gly296
Accession # Q8CCG4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse DPPA4
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry DPPA4 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

DPPA4 in D3 Mouse Cell Line. DPPA4 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3730) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA4

DPPA4 is a 33 kDa molecule that is expressed specifically in the early embryo, uncommitted cells of the germ line, and embryonic stem cells and is used as a marker of pluripotent cells. Mouse DPPA4 is 296 amino acids in length and contains an SAP (SAF-A/B, ACINUS, PIAS) putative DNA-binding domain. Two potential isoforms exist with deletions of amino acids 104‑120 and 69‑120, respectively. In the region used as an immunogen, mouse DPPA4 shows 57% amino acid sequence identity with human and 87% identity with rat DPPA4 proteins.

Long Name
Developmental Pluripotency Associated 4
Entrez Gene IDs
55211 (Human); 73693 (Mouse)
Alternate Names
developmental pluripotency associated 4; developmental pluripotency-associated protein 4; DPPA4; FLJ10713,2410091M23Rik

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Citations for Mouse DPPA4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  2. Mitotic Arrest in Teratoma Susceptible Fetal Male Germ Cells
    Authors: Patrick S. Western, Rachael A. Ralli, Stephanie I. Wakeling, Camden Lo, Jocelyn A. van den Bergen, Denise C. Miles et al.
    PLoS ONE
  3. Multimodal characterization of murine gastruloid development
    Authors: Simon Suppinger, Marietta Zinner, Nadim Aizarani, Ilya Lukonin, Raphael Ortiz, Chiara Azzi et al.
    Cell Stem Cell
  4. Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells
    Authors: I Theurillat, IA Hendriks, JC Cossec, A Andrieux, ML Nielsen, A Dejean
    Cell Rep, 2020-09-15;32(11):108146.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Tox4 modulates cell fate reprogramming
    Authors: L Vanheer, J Song, N De Geest, A Janiszewsk, I Talon, C Provenzano, T Oh, J Chappell, V Pasque
    J. Cell. Sci., 2019-10-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance
    Authors: JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western
    BMC Biol., 2018-09-20;16(1):104.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer
    Authors: RH Klein, PY Tung, P Somanath, HJ Fehling, PS Knoepfler
    Stem Cell Res, 2018-07-19;31(0):83-94.
    Species: Mouse
    Sample Types: Protein
    Applications: ChIP
  8. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency
    Authors: K Adachi, W Kopp, G Wu, S Heising, B Greber, M Stehling, MJ Araúzo-Bra, ST Boerno, B Timmermann, M Vingron, HR Schöler
    Cell Stem Cell, 2018-06-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  9. Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency
    Authors: M Sone, N Morone, T Nakamura, A Tanaka, K Okita, K Woltjen, M Nakagawa, JE Heuser, Y Yamada, S Yamanaka, T Yamamoto
    Cell Metab., 2017-05-02;25(5):1103-1117.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes.
    Authors: Tung P, Varlakhanova N, Knoepfler P
    Stem Cells, 2013-11-01;31(11):2330-42.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  11. Maternal Dppa2 and Dppa4 are dispensable for zygotic genome activation but important for offspring survival
    Authors: Oana Kubinyecz, Fatima Santos, Deborah Drage, Wolf Reik, Melanie A. Eckersley-Maslin
    Development
  12. Signaling through the TGF Beta-Activin Receptors ALK4/5/7 Regulates Testis Formation and Male Germ Cell Development
    Authors: Denise C. Miles, Stephanie I. Wakeling, Jessica M. Stringer, Jocelyn A. van den Bergen, Dagmar Wilhelm, Andrew H. Sinclair et al.
    PLoS ONE

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Mouse DPPA4 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Mouse iPS Species: Mouse

Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:250

Immunocytochemistry/Immunofluorescence Mouse DPPA4 Antibody AF3730