Mouse E-Selectin/CD62E Antibody Summary
Trp22-Pro557
Accession # Q00690
Applications
under non-reducing conditions only
Mouse E-Selectin/CD62E Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E‑Selectin proteins share 81% amino acid similarity.
E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
Product Datasheets
Citations for Mouse E-Selectin/CD62E Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Immunohistochemical study for the expression of leukocyte adhesion molecules, and FGF23 and ACE2 in P. gingivalis LPS-induced diabetic nephropathy
Authors: K Kajiwara, Y Sawa, T Fujita, S Tamaoki
Bmc Nephrology, 2021-01-06;22(1):3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Glycoengineering of Chimeric Antigen Receptor (CAR) T-cells to Enforce E-selectin Binding
Authors: N Mondal, M Silva, AP Castano, MV Maus, R Sackstein
J. Biol. Chem., 2019-10-18;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow
Authors: M De Niz, E Meibalan, P Mejia, S Ma, NMB Brancucci, C Agop-Nerse, R Mandt, P Ngotho, KR Hughes, AP Waters, C Huttenhowe, JR Mitchell, R Martinelli, F Frischknec, KB Seydel, T Taylor, D Milner, VT Heussler, M Marti
Sci Adv, 2018-05-23;4(5):eaat3775.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Authors: Kato N, Yuzawa Y, Kosugi T
J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
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