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Mouse Endoglin/CD105 Antibody

Catalog #: AF1320
18 Citations 1 Review Datasheet / COA / SDS
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AF1320
AF1320-SP
Detection of Mouse Endoglin/CD105 by Western Blot.
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Product Details
Citations (18)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endoglin/CD105 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat Endoglin is observed and less than 5% cross-reactivity with recombinant human Endoglin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
Glu27-Gly581
Accession # Q8K100
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
MS-1 mouse cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Endoglin/CD105 antibody by Western Blot. View Larger

Detection of Mouse Endoglin/CD105 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and MS-1 mouse pancreatic islet endothelial cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Endoglin/CD105 antibody in MS-1 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Endoglin/CD105 in MS-1 Mouse Cell Line. Endoglin/CD105 was detected in immersion fixed MS-1 mouse pancreatic islet endothelial cell line using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Endoglin/CD105 antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Endoglin/CD105 in Rat Mesenchymal Stem Cells. Endoglin/CD105 was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Endoglin/CD105 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (E13-15) using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Endoglin/CD105 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Endoglin/CD105 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Mouse Endoglin/CD105 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Mitochondrial respiration supports autophagy to provide stress resistance during quiescence
    Authors: S Magalhaes-, J Blecha, R Naraine, J Mikesova, P Abaffy, A Pecinova, M Milosevic, R Bohuslavov, J Prochazka, S Khan, E Novotna, R Sindelka, R Machan, M Dewerchin, E Vlcak, J Kalucka, S Stemberkov, A Benda, J Goveia, T Mracek, C Barinka, P Carmeliet, J Neuzil, K Rohlenova, J Rohlena
    Autophagy, 2022-03-08;0(0):1-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Bevacizumab dose adjustment to improve clinical outcomes of glioblastoma
    Authors: N García-Rom, I Palacín-Al, R Madurga, J Carrión-Na, S Esteban-Ru, B Jiménez, A Collazo, F Pérez-Rodr, A Ortiz de M, C Fernández-, S García-Duq, J Diamantopo, C Belda-Inie, R Prat-Acín, P Sánchez-Gó, E Calvo, A Ayuso-Saci
    BMC Med, 2020-06-22;18(1):142.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival
    Authors: Y Yoon, G Voloudakis, N Doran, E Zhang, C Dimovasili, L Chen, Z Shao, S Darmanis, C Tang, J Tang, VX Wang, PR Hof, NK Robakis, A Georgakopo
    Mol. Psychiatry, 2020-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors
    Authors: DD He, XT Tang, W Dong, G Cui, G Peng, X Yin, Y Chen, N Jing, BO Zhou
    Stem Cell Reports, 2020-03-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Correlation of two distinct metastasis-associated proteins, MTA1 and S100A4, in angiogenesis for promoting tumor growth
    Authors: M Ishikawa, M Osaki, M Yamagishi, K Onuma, H Ito, F Okada, H Endo
    Oncogene, 2019-02-11;0(0):.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Role of glutamine synthetase in angiogenesis beyond glutamine synthesis
    Authors: G Eelen, C Dubois, AR Cantelmo, J Goveia, U Brüning, M DeRan, G Jarugumill, J van Rijsse, G Saladino, F Comitani, A Zecchin, S Rocha, R Chen, H Huang, S Vandekeere, J Kalucka, C Lange, F Morales-Ro, B Cruys, L Treps, L Ramer, S Vinckier, K Brepoels, S Wyns, J Souffreau, L Schoonjans, WH Lamers, Y Wu, J Haustraete, J Hofkens, S Liekens, R Cubbon, B Ghesquière, M Dewerchin, FL Gervasio, X Li, JD van Buul, X Wu, P Carmeliet
    Nature, 2018-08-29;561(7721):63-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, IHC-Fr
  7. Blockade of myeloid-derived suppressor cell expansion with all-trans retinoic acid increases the efficacy of anti-angiogenic therapy
    Authors: R Bauer, F Udonta, M Wroblewski, I Ben-Batall, IM Santos, F Taverna, M Kuhlencord, V Gensch, S Päsler, S Vinckier, JM Brandner, K Pantel, C Bokemeyer, T Vogl, J Roth, P Carmeliet, S Loges
    Cancer Res., 2018-04-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B
    Authors: M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges
    Nat Commun, 2017-08-16;8(1):269.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis
    Authors: EM Palmieri, A Menga, R Martín-Pér, A Quinto, C Riera-Domi, G De Tullio, DC Hooper, WH Lamers, B Ghesquière, DW McVicar, A Guarini, M Mazzone, A Castegna
    Cell Rep, 2017-08-15;20(7):1654-1666.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. DNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients
    Authors: N García-Rom, J Carrión-Na, S Esteban-Ru, E Lázaro-Ibá, M Peris-Celd, MM Alonso, J Guzmán-De-, C Fernández-, AO de Mendivi, S García-Duq, C Escobedo-L, R Prat-Acín, C Belda-Inie, A Ayuso-Saci
    Oncotarget, 2017-01-03;8(1):1416-1428.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
    Nat Commun, 2016-11-29;7(0):13650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Gene Electrotransfer of Plasmid with Tissue Specific Promoter Encoding shRNA against Endoglin Exerts Antitumor Efficacy against Murine TS/A Tumors by Vascular Targeted Effects.
    Authors: Stimac M, Dolinsek T, Lampreht U, Cemazar M, Sersa G
    PLoS ONE, 2015-04-24;10(4):e0124913.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.
    Authors: Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B
    PLoS ONE, 2014-01-15;9(1):e85655.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. In vivo adeno-associated viral vector-mediated genetic engineering of white and brown adipose tissue in adult mice.
    Authors: Jimenez V, Munoz S, Casana E, Mallol C, Elias I, Jambrina C, Ribera A, Ferre T, Franckhauser S, Bosch F
    Diabetes, 2013-09-16;62(12):4012-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Multiple delivery of siRNA against endoglin into murine mammary adenocarcinoma prevents angiogenesis and delays tumor growth.
    Authors: Dolinsek T, Markelc B, Sersa G, Coer A, Stimac M, Lavrencak J, Brozic A, Kranjc S, Cemazar M
    PLoS ONE, 2013-03-05;8(3):e58723.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  17. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2012-12-21;190(3):1094-102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010-04-01;120(5):1694-707.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Mouse Endoglin/CD105 Antibody
By Anonymous on 05/16/2017
Application: ICC/IF Sample Tested: Embryonic heart Species: Mouse

Block - 5% secondary specific serum + 1% BSA + 0.5% Tween-20 - 2hrs at RT
Primary Ab - diluted in block - 1 in 25 - for O/N at 4'C
Secondary ab - diluted in block - 2hrs at RT

Immunocytochemistry/Immunofluorescence Mouse Endoglin/CD105 Antibody AF1320