Mouse Endoglin/CD105 Biotinylated Antibody

Catalog #: BAF1320
4 Citations Datasheet / COA / SDS

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BAF1320

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endoglin/CD105 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endoglin/CD105 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human Endoglin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
Glu27-Gly581
Accession # Q8K100

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN)

Flow Cytometry

0.25 µg/10⁶ cells

MS-1 mouse cell line

Immunohistochemistry

5-15 µg/mL

See below

Mouse Endoglin/CD105 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Endoglin/CD105 Antibody (Catalog # MAB13201)

Standard: Recombinant Mouse Endoglin/CD105 Fc Chimera Protein, CF (Catalog # 1320-EN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Endoglin/CD105 in Mouse Lung. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse lung using 15 µg/mL Goat Anti-Mouse Endoglin/CD105 Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1320) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Flow Cytometry

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Detection of Endoglin/CD105 in MS-1 Mouse Cell Line by Flow Cytometry. MS-1 mouse cell line was stained with Goat Anti-Mouse Endoglin/CD105 Biotinylated Polyclonal Antibody (Catalog # BAF1320, filled histogram) or control antibody (BAF108, open histogram), followed by Phycoerythrin-conjugated Streptavidin (F0040). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References

Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
ten Dijke, P. et al. (2008) Angiogenesis 11:79.
Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
Moody, J.L. et al. (2007) Stem Cells 25:2809.
Velasco, S. et al. (2008) J. Cell Sci. 121:913.
Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.
Venkatesha, S. et al. (2006) Nat. Med. 12:642.

Entrez Gene IDs

2022 (Human); 13805 (Mouse); 497010 (Rat)

Alternate Names

CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citations for Mouse Endoglin/CD105 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats
Authors: Z Berkova, K Zacharovov, A Patikova, I Leontovyc, Z Hladikova, D Cerveny, E Tihlarikov, V Nedela, P Girman, D Jirak, F Saudek
Journal of functional biomaterials, 2022-09-30;13(4):.
Species: Rat
Sample Types: Whole Cell
Applications: Flow Cytometry
Targeting Endoglin Expressing Cells in the Tumor Microenvironment Does Not Inhibit Tumor Growth in a Pancreatic Cancer Mouse Model
Authors: MJA Schoonderw, SK Hakuno, M Sassen, EB Kuhlemaije, M Paauwe, M Slingerlan, MF Fransen, LJAC Hawinkels
OncoTargets and Therapy, 2021-10-29;14(0):5205-5220.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Long-Term Retinal PEDF Overexpression Prevents Neovascularization in a Murine Adult Model of Retinopathy.
Authors: Haurigot V, Villacampa P, Ribera A, Bosch A, Ramos D, Ruberte J, Bosch F
PLoS ONE, 2012-07-20;7(7):e41511.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Endoglin expression in the endothelium is regulated by Fli-1, Erg, and Elf-1 acting on the promoter and a -8-kb enhancer.
Authors: Pimanda JE, Chan WY, Donaldson IJ, Bowen M, Green AR, Gottgens B
Blood, 2006-02-16;107(12):4737-45.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC