Mouse EphA7 Antibody

Name: Mouse EphA7 Antibody
Brand: R&D Systems
SKU: AF608
Rating: 5 (2 reviews)

Catalog #: AF608 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF608
AF608-SP

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Mouse EphA7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse EphA7 in direct ELISAs and Western blots. In direct ELISAs, approximately 2% cross‑reactivity with recombinant mouse (rm) EphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, and rmEphA8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphA7 isoform 1
Ala30-Pro554 (Gly540-Glu544 del)
Accession # Q61772

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse EphA7 Fc Chimera (Catalog # 608-A7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA7

EphA7, also known as Mdk1, Hek11, Ehk3, Ebk, and Cek11 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA7 has been shown to bind ephrin-A2, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-A5 (2, 3). The extracellular domains of human and mouse EphA7 share greater than 97% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

Eph Nomenclature Committee [letter] (1997) Cell 90:403.
Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Long Name

Eph Receptor A7

Entrez Gene IDs

2045 (Human); 13841 (Mouse)

Alternate Names

Cek11; Ebk; EC 2.7.10; EC 2.7.10.1; Ehk3; EHK-3; EK11; EPH homology kinase 3; Eph homology kinase-3; EPH receptor A7; EphA7; EPH-like kinase 11; ephrin type-A receptor 7; Hek11; Mdk1; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3