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Mouse EphB4 Antibody

Catalog #: AF446
34 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF446
AF446-SP
EphB4 in Mouse Embryo.
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Product Details
Citations (34)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EphB4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB4
Leu16-Ala539
Accession # P54761
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EphB4 Fc Chimera (Catalog # 446-B4)
Flow Cytometry
2.5 µg/106 cells
MCF‑7 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry EphB4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

EphB4 in Mouse Embryo. EphB4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse EphB4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF446) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin‑B2 and ephrin‑B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name
Eph Receptor B4
Entrez Gene IDs
2050 (Human); 13846 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

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Citations for Mouse EphB4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Genetic dysregulation of an endothelial Ras signaling network in vein of Galen malformations
    Authors: S Zhao, KY Mekbib, MA van der En, G Allington, A Prendergas, JE Chau, H Smith, J Shohfi, J Ocken, D Duran, CG Furey, HT Le, PQ Duy, BC Reeves, J Zhang, C Nelson-Wil, D Chen, B Li, T Nottoli, S Bai, M Rolle, X Zeng, W Dong, PY Fu, YC Wang, S Mane, P Piwowarczy, KP Fehnel, AP See, BJ Iskandar, B Aagaard-Ki, AJ Kundishora, T DeSpenza, ABW Greenberg, SM Kidanemari, AT Hale, JM Johnston, EM Jackson, PB Storm, SS Lang, WE Butler, BS Carter, P Chapman, CJ Stapleton, AB Patel, G Rodesch, S Smajda, A Berenstein, T Barak, EZ Erson-Omay, H Zhao, A Moreno-De-, MR Proctor, ER Smith, DB Orbach, SL Alper, S Nicoli, TJ Boggon, RP Lifton, M Gunel, PD King, SC Jin, KT Kahle
    bioRxiv : the preprint server for biology, 2023-03-21;0(0):.
    Species: Zebrafish
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
    Authors: S Kohara, K Ogawa
    Biomedicines, 2022-12-12;10(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. Histochemical examination of blood vessels in murine femora with intermittent PTH administration
    Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
    Journal of oral biosciences, 2022-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting
    Authors: AE Lewis, A Kuwahara, J Franzosi, JO Bush
    Cell Reports, 2022-03-15;38(11):110510.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  5. Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary
    Authors: J Alam, K Ogawa
    Reproductive biology, 2021-05-13;21(3):100511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells
    Authors: C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto
    Cell Rep, 2020-09-08;32(10):108105.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Endothelial EphB4 maintains vascular integrity and transport function in adult heart
    Authors: G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams
    Elife, 2019-11-29;8(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart
    Authors: S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val
    Nat Commun, 2019-07-22;10(1):3276.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Venous identity requires BMP signalling through ALK3
    Authors: A Neal, S Nornes, S Payne, MD Wallace, M Fritzsche, P Louphrasit, RN Wilkinson, KM Chouliaras, K Liu, K Plant, R Sholapurka, I Ratnayaka, W Herzog, G Bond, T Chico, G Bou-Ghario, S De Val
    Nat Commun, 2019-01-28;10(1):453.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  10. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
    Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
    EMBO Rep., 2018-04-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
    Authors: H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li
    Biomed. Res., 2017-01-01;38(2):123-134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016-07-29;7(0):12329.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol
    J Exp Clin Cancer Res, 2016-07-22;35(1):118.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis
    J Clin Invest, 2016-07-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase
    Authors: M Wang, MJ Collins, TR Foster, H Bai, T Hashimoto, JM Santana, C Shu, A Dardik
    J. Vasc. Surg., 2016-01-24;65(1):179-189.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
    Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
    Stem Cells, 2015-06-23;33(9):2838-49.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015-04-13;6(0):6625.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice.
    Authors: Valverde-Franco G, Hum D, Matsuo K, Lussier B, Pelletier J, Fahmi H, Kapoor M, Martel-Pelletier J
    Am J Pathol, 2014-11-29;185(2):335-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Single-cell western blotting.
    Authors: Hughes A, Spelke D, Xu Z, Kang C, Schaffer D, Herr A
    Nat Methods, 2014-06-01;11(7):749-55.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals.
    Authors: Lindskog, Henrik, Kim, Yung Hae, Jelin, Eric B, Kong, Yupeng, Guevara-Gallardo, Salvador, Kim, Tyson N, Wang, Rong A
    Development, 2014-03-01;141(5):1120-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Sox17 is required for normal pulmonary vascular morphogenesis.
    Authors: Lange A, Haitchi H, LeCras T, Sridharan A, Xu Y, Wert S, James J, Udell N, Thurner P, Whitsett J
    Dev Biol, 2014-01-10;387(1):109-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
    Authors: Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
    Eur. J. Immunol., 2012-05-23;42(6):1562-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  23. Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
    Authors: Muto A, Yi T, Harrison KD, Davalos A, Fancher TT, Ziegler KR, Feigel A, Kondo Y, Nishibe T, Sessa WC, Dardik A
    J. Exp. Med., 2011-02-21;208(3):561-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. EphB-ephrin-B2 interactions are required for thymus migration during organogenesis.
    Authors: Foster KE, Gordon J, Cardenas K
    Proc. Natl. Acad. Sci. U.S.A., 2010-07-08;107(30):13414-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.
    Authors: Wang Y, Nakayama M, Pitulescu ME, Schmidt TS, Bochenek ML, Sakakibara A, Adams S, Davy A, Deutsch U, Luthi U, Barberis A, Benjamin LE, Makinen T, Nobes CD, Adams RH
    Nature, 2010-05-27;465(7297):483-6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010-04-01;120(5):1694-707.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice.
    Authors: Xie J, Wu T, Xu K, Huang IK, Cleaver O, Huang CL
    Am. J. Pathol., 2009-07-30;175(3):1315-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis.
    Authors: Kim YH, Hu H, Guevara-Gallardo S, Lam MT, Fong SY, Wang RA
    Development, 2008-11-01;135(22):3755-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  29. EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
    Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
    J. Bone Miner. Res., 2008-08-01;23(8):1170-81.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
    Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
    Biol. Reprod., 2008-05-07;79(3):450-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006-08-31;281(43):32574-86.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Immunoprecipitation, Western Blot
  32. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006-02-01;119(0):559-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Inhibition of tumor growth and angiogenesis by soluble EphB4.
    Authors: Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG
    Neoplasia, 2004-05-01;6(3):248-57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
    Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
    J. Cell. Sci., 2003-05-06;116(0):2461-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse EphB4 Antibody
By Anonymous on 12/16/2020
Application: ICC/IF Sample Tested: E12.5 mouse embryo fixed in 4% PFA Species: Mouse

Dilution used - 1:200. The staining was done on an E12.5 transverse (4% PFA fixed) mouse section using standard IF techniques.
Used 1% BSA for blocking for 1 hour before adding the primary antibody.
The results are hit or miss for me, because I have noticed expression on non target tissue (Sometimes it doesn't appear, sometimes it does).
Attached pictures shows a artery (smaller circle) and a vein (bigger circle), and while the EphB4 expression on the vein is very good (as it is supposed to express there), there seems to be some non-specific expression on the adjacent artery as well.

Immunocytochemistry/Immunofluorescence Mouse EphB4 Antibody AF446
Mouse EphB4 Antibody
By Anonymous on 11/13/2020
Application: WB Sample Tested: embryos Species: Zebrafish
Western Blot Mouse EphB4 Antibody AF446