Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: ErbB2/Her2

ErbB2, also known as CD340, Neu, and Her2 (Human Epidermal Growth Factor Receptor 2), is a 180-185 kDa type I transmembrane glycoprotein that is a member of the Class I family of tyrosine kinase receptors. ErbB2 is widely expressed on and in epithelial cells, and plays a role in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (ECD, amino acids 23-653) contains two "L" domains and two cystine-rich domains.  Within the ECD, it shares 85% and 94% amino acid sequence identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 95-110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 is also known to undergo alternative splicing, and to appear in the nucleus as part of a transcriptional regulatory complex.  ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. It also appears to form a complex with Plexin B1, c-met, and Mucin-4.  Within those complexes, non-EGF ligands are likely involved.  The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Cancer Biomarkers
• Cancer Stem Cell Markers
• Cytokine and Growth Factor Receptors on VSMC
• EGF Family
• Receptor Tyrosine Kinases (RTKs)
• Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
• Receptors in the Jak/STAT Pathway
• Semaphorins, Plexin Receptors, and Related Molecules