Mouse ErbB2/Her2 Antibody

Catalog #: AF5176
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5176
AF5176-SP

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Mouse ErbB2/Her2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ErbB2/Her2 in direct ELISAs and Western blots. In Western blots using recombinant protein, approximately 10% cross‑reactivity with recombinant human ErbB2 is observed and less than 1% cross‑reactivity with recombinant mouse (rm) ErbB3 and rmErbB4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
Thr23-Thr653
Accession # P70424

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

3T3‑L1 mouse embryonic fibroblast adipose-like cell line.

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ErbB2/Her2 in Mouse Intestine. ErbB2/Her2 was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Mouse ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5176) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a 185 kDa type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (amino acids 23-653 of 1256) shares 85% and 94% aa identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Alternate Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse ErbB2/Her2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Soluble SORLA enhances neurite outgrowth and regeneration through activation of the EGF Receptor/ERK signaling axis
Authors: J Stupack, XP Xiong, LL Jiang, T Zhang, L Zhou, A Campos, B Ranscht, W Mobley, EB Pasquale, H Xu, TY Huang
J. Neurosci., 2020-06-29;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot