Mouse ErbB3/Her3 Antibody

Catalog #: AF4518 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4518
AF4518-SP

Detection of ErbB3/Her3 in C2C12 Mouse Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ErbB3/Her3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ErbB3/Her3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human ErbB3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ErbB3/Her3
Ser20-His641
Accession # Q61526

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ErbB3/Her3 Fc Chimera (Catalog # 4518-RB)

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ErbB3/Her3 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Sheep Anti-Mouse ErbB3/Her3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4518, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Immunocytochemistry

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ErbB3/Her3 in MCF‑7 and MDA‑MD‑231 Human Cell Lines. ErbB3/Her3 was detected in immersion fixed MCF-7 human breast cancer cell line (left panel) and MDA-MD-231 human breast cancer cell line (right panel) using Sheep Anti-Mouse ErbB3/Her3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4518) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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ErbB3/Her3 in Embryonic Mouse Dorsal Root Ganglion. ErbB3/Her3 was detected in immersion fixed frozen sections of embryonic mouse dorsal root ganglion (E10.5) using 10 µg/mL Sheep Anti-Mouse ErbB3/Her3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4518) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors named for a viral oncogene (1‑3). ErbB family members serve as receptors for the EGF family of growth factors (1‑3). Mouse ErbB3 contains a 19 amino acid (aa) signal sequence, a 622 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 677 aa cytoplasmic domain (4). Human ErbB3 has four isoforms created by intron read-through and truncation of the molecule (5). Three of these are secreted and at least one can inhibit ErbB3 activity (6). Little information is available concerning mouse ErbB3 isoforms. The mouse ErbB3 ECD shares 97%, 93%, 92%, 91%, 89% and 88% aa identity with rat, human, bovine, equine, canine and opossum ErbB3, respectively. ErbB3 is found in epithelial cell layers of gastrointestinal, reproductive, urinary, endocrine and nervous systems, skin and muscle (3). Among ErbB family members, only ErbB3 lacks a working kinase domain, requiring heterodimerization with another ErbB receptor for signaling (1‑3). The heterodimer of ErbB3 with ErbB2, which has no known ligands of its own, is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2 (3, 7‑9). These ligands also bind ErbB4 (1). Signaling is aided by the six consensus binding motifs for the SH2 domain and one for the SH3 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (10, 11). Deletion studies in mice demonstrate non-redundant roles for ErbB3 in development of Schwann cells, neural crest cells and heart valves (12, 13). ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system (14).

References

Linggi, B. and G. Carpenter (2006) Trends Cell Biol. 16:649.
Citri, A. and Y. Yarden (2006) Nat. Rev. Mol. Cell Biol. 7:505.
Citri, A. et al. (2003) Exp. Cell Res. 284:54.
Swissprot Accession # Q61526.
Lee, H. et al. (1998) Oncogene 16:3243.
Lee, H. et al. (2001) Cancer Res. 61:4467.
Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
Sundaresan, S. et al. (1998) Endocrinology 139:4756.
Wallasch, C. et al. (1995) EMBO J. 14:4267.
Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
Riethmacher, D. et al. (1997) Nature 389:725.
Erickson, S.L. et al. (1997) Development 124:4999.
Britsch, S. et al. (1998) Genes Dev. 12:1825.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Alternate Names

c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)