Home / F4/80 / Mouse F4/80 Antibody

Mouse F4/80 Antibody

Catalog #: MAB5580
17 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5580
MAB5580-SP
Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (17)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F4/80 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse F4/80/EMR1.
Source
Monoclonal Rat IgG2A Clone # 521204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed and unfixed live RAW 264.7 mouse monocyte/macrophage cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Immunocytochemistry View Larger

F4/80 in RAW 264.7 mouse monocyte/macrophage cell line. F4/80 was detected in unfixed live cells fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Entrez Gene IDs
13733 (Mouse); 316137 (Rat)
Alternate Names
ADGRE1; cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; EMR1 hormone receptor; Emr1; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7L; TM7LN3

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Citations for Mouse F4/80 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Schwann cell-derived CXCL2 contributes to cancer pain by modulating macrophage infiltration in a mouse breast cancer model
    Authors: Y Zhang, R Sang, J Bao, Z Jiang, D Qian, Y Zhou, W Su, J Wei, L Zhao, Z Wei, Y Zhao, M Shi, G Chen
    Brain, Behavior, and Immunity, 2023-02-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Melatonin Suppresses Macrophage M1 Polarization and ROS-Mediated Pyroptosis via Activating ApoE/LDLR Pathway in Influenza A-Induced Acute Lung Injury
    Authors: MM Xu, JY Kang, S Ji, YY Wei, SL Wei, JJ Ye, YG Wang, JL Shen, HM Wu, GH Fei
    Oxidative Medicine and Cellular Longevity, 2022-11-15;2022(0):2520348.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation
    Authors: MS Lee, J Jeon, S Park, J Lim, HS Yang
    Oncogene, 2022-06-16;20(0):368-380.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction
    Authors: L Zhang, J Chen, L Yan, Q He, H Xie, M Chen
    Frontiers in Pharmacology, 2021-06-10;12(0):646240.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Interleukin-22 deficiency alleviates doxorubicin-induced oxidative stress and cardiac injury via the p38 MAPK/macrophage/Fizz3 axis in mice
    Authors: J Ye, Y Wang, Y Xu, Z Wang, L Liu, M Wang, D Ye, J Zhang, Z Yang, Y Lin, Q Ji, J Wan
    Redox Biol, 2020-07-07;36(0):101636.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Heparin inhibits pro-inflammatory and promotes anti-inflammatory macrophage polarization under hyperglycemic stress
    Authors: A Abbadi, J Loftis, A Wang, M Yu, Y Wang, S Shakya, X Li, EV Maytin, VC Hascall
    J. Biol. Chem., 2020-02-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis
    Authors: H Tanaka, K Horioka, M Yamamoto, M Asari, K Okuda, K Yamazaki, K Shimizu, K Ogawa
    Cancer Sci., 2019-07-30;110(9):2748-2759.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Osteopontin in the host response to Leishmania amazonensis
    Authors: E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou
    BMC Microbiol., 2019-02-08;19(1):32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Self-implantable double-layered micro-drug-reservoirs for efficient and controlled ocular drug delivery
    Authors: A Than, C Liu, H Chang, PK Duong, CMG Cheung, C Xu, X Wang, P Chen
    Nat Commun, 2018-11-06;9(1):4433.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment
    Authors: J Zhou, J Li, Y Yu, Y Liu, H Li, Y Liu, J Wang, L Zhang, X Lu, Z Chen, D Zuo
    J. Leukoc. Biol., 2018-10-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function
    Authors: L Zhuang, Y Jang, YK Park, JE Lee, S Jain, E Froimchuk, A Broun, C Liu, O Gavrilova, K Ge
    Nat Commun, 2018-05-04;9(1):1796.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Central role of myeloid MCPIP1 in protecting against LPS-induced inflammation and lung injury
    Authors: Y Li, X Huang, S Huang, H He, T Lei, F Saaoud, XQ Yu, A Melnick, A Kumar, CJ Papasian, D Fan, M Fu
    Signal Transduct Target Ther, 2017-12-08;2(0):17066.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells.
    Authors: Maria Fe Lanfran, David J Loane, Italo Mocchetti, Mark P Burns, Sonia Villapol
    Bio-Protocol, 2017-11-20;0(0):2331-8325.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
    Proc Natl Acad Sci USA, 2016-05-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity.
    Authors: Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A
    2013-03-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012-08-10;9(0):193.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012-06-13;5(0):702.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse F4/80 Antibody
By Anonymous on 12/01/2017
Application: IHC Sample Tested: Colon tissue Species: Mouse

We tried 4 difference sources and none of them, including this one, works. Our experience is that no F4/80 antibody works for FFPE samples. Although it is not uncommon to see published positive staining, those data using FFPE samples cannot be trusted.

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