Home / F4/80 / Mouse F4/80 PE-conjugated Antibody

Mouse F4/80 PE-conjugated Antibody

Catalog #: FAB5580P
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5580P-100UG
Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F4/80 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse F4/80/EMR1.
Source
Monoclonal Rat IgG2A Clone # 521204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: F4/80

F4/80, also known as EMR1 in humans, is a 160 kDa glycosylated protein that belongs to the EGF-TM7 family of adhesion molecules. It is expressed on splenic red pulp and bone marrow, macrophages, microglia, Langerhans cells, monocytes, eosinophils, and CD116+ dendritic cells in mouse, but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance, being necessary for the development of CD8+ T regs. It also serves as a marker for IL-1 beta + proinflammatory macrophages.

Entrez Gene IDs
13733 (Mouse); 316137 (Rat)
Alternate Names
ADGRE1; cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; EMR1 hormone receptor; Emr1; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7L; TM7LN3

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Citations for Mouse F4/80 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Tumor-Released Products Promote Bone Marrow-Derived Macrophage Survival and Proliferation
    Authors: JM Motta, VM Rumjanek, A Mantovani, M Locati
    Biomedicines, 2021-10-04;9(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Functional loss of inactive rhomboid-like protein�2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation
    Authors: M Xu, C Ge, Y Qin, D Lou, Q Li, J Feng, Y Wu, L Hu, B Wang, J Tan
    Mol Metab, 2020-01-22;34(0):112-123.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK-NF-?B signaling mediated inflammation
    Authors: J Ben, B Jiang, D Wang, Q Liu, Y Zhang, Y Qi, X Tong, L Chen, X Liu, Y Zhang, X Zhu, X Li, H Zhang, H Bai, Q Yang, J Ma, EAC Wiemer, Y Xu, Q Chen
    Nat Commun, 2019-04-17;10(1):1801.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection.
    Authors: Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T
    J Immunol, 2015-03-11;194(8):3861-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Galectin-3 negatively regulates the frequency and function of CD4(+) CD25(+) Foxp3(+) regulatory T cells and influences the course of Leishmania major infection.
    Authors: Fermino M, Dias F, Lopes C, Souza M, Cruz A, Liu F, Chammas R, Roque-Barreira M, Rabinovich G, Bernardes E
    Eur J Immunol, 2013-05-17;43(7):1806-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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