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Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

Catalog #: AF1460
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1460
AF1460-SP
Fc gamma RII/RIII (CD32/CD16) in Mouse Splenocytes.
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Citations (10)
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Mouse Fc gamma RII/RIII (CD32/CD16) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Fc gamma RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # P08101
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Fc? RII/RIII (CD32/CD16) antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Fc gamma RII/RIII (CD32/CD16) in Mouse Splenocytes. Fc gamma RII/RIII (CD32/CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RII/RIII (CD32/CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1460) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Long Name
Fc gamma Receptor II/III
Alternate Names
CD16;CD16A;CD16-II;FCG3;FCGR3;FCGR3A;FCGRIII;FcGRIIIA;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20;Low affinity immunoglobulin gamma Fc region receptor III-A; CD32/CD16; Fc gamma RII/RIII

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Citations for Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Motor neuron survival is associated with reduced neuroinflammation and increased autophagy after brachial plexus avulsion injury in aldose reductase-deficient mice
    Authors: K Zhong, Y Huang, PLM Zilundu, Y Wang, Y Zhou, G Yu, R Fu, SK Chung, Y Tang, X Cheng, L Zhou
    Journal of Neuroinflammation, 2022-11-09;19(1):271.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. SENP6 induces microglial polarization and neuroinflammation through de-SUMOylation of Annexin-A1 after cerebral ischaemia-reperfusion injury
    Authors: M Mao, Q Xia, GF Zhan, QJ Chu, X Li, HK Lian
    Cell & bioscience, 2022-07-22;12(1):113.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment
    Authors: SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud
    Journal of Translational Medicine, 2022-02-02;20(1):62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  4. Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation
    Authors: J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch
    Nature Communications, 2021-11-29;12(1):6963.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease mice
    Authors: Y Wang, Y Lin, L Wang, H Zhan, X Luo, Y Zeng, W Wu, X Zhang, F Wang
    Aging (Albany NY), 2020-10-16;12(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  6. FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophages
    Authors: D Wang, F Liu, L Zhu, P Lin, F Han, X Wang, X Tan, L Lin, Y Xiong
    J Neuroinflammation, 2020-08-31;17(1):257.
    Species: Mouse
    Sample Types: Whole Tissue, Whole Tissues
    Applications: IF, IHC
  7. Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress
    Authors: P Ren, J Chen, B Li, M Zhang, B Yang, X Guo, Z Chen, H Cheng, P Wang, S Wang, N Wang, G Zhang, X Wu, D Ma, D Guan, R Zhao
    Oxid Med Cell Longev, 2020-05-11;2020(0):3050971.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. A purified capsular polysaccharide markedly inhibits inflammatory response during endotoxic shock.
    Authors: Piccioni M, Monari C, Kenno S, Pericolini E, Gabrielli E, Pietrella D, Perito S, Bistoni F, Kozel T, Vecchiarelli A
    Infect Immun, 2012-10-22;81(1):90-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011-07-09;54(10):2713-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. New asthma biomarkers: lessons from murine models of acute and chronic asthma.
    Authors: Di Valentin E, Crahay C, Garbacki N, Hennuy B, Gueders M, Noel A, Foidart JM, Grooten J, Colige A, Piette J, Cataldo D
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-11-21;296(2):L185-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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