Human/Mouse Frizzled-1 Biotinylated Antibody

Catalog #: BAM11201
1 Citation Datasheet / COA / SDS

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BAM11201

All Frizzled-1 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Frizzled-1 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Frizzled-1 in direct ELISAs and Western blots and detects human Frizzled-1 by flow cytometry. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.

Source

Monoclonal Rat IgG_2A Clone # 162531

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-1 Gln1-His248 (Met122Ile) Accession # O70421

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Frizzled-1 Fc Chimera
Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 human embryonic kidney cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Frizzled-1

Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Alternate Names

DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse Frizzled-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Temporal Regulation of Natural Killer T Cell Interferon Gamma Responses by Î²-Catenin-Dependent and -Independent Wnt Signaling
Authors: JC Kling, MA Jordan, LA Pitt, J Meiners, T Thanh-Tran, LS Tran, TTK Nguyen, D Mittal, R Villani, RJ Steptoe, K Khosrotehr, SP Berzins, AG Baxter, DI Godfrey, A Blumenthal
Front Immunol, 2018-03-16;9(0):483.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry