Mouse Frizzled-7 Biotinylated Antibody

Catalog #: BAF198 Datasheet / COA / SDS

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BAF198

All Frizzled-7 Antibodies

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Mouse Frizzled-7 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Frizzled-7 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse (rm) Frizzled-2 is observed and less than 2% cross-reactivity with rmFrizzled-3, rmFrizzled-4, recombinant human Frizzled-5, rmFrizzled-6, rmFrizzled-8, and rmFrizzled-9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Frizzled‑7 Fc Chimera (Catalog # 198-FZ)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises the Wnt binding site, spans about 130 amino acid residues and contains ten invariant cysteine residues. Mouse Frizzled-7 contains 572 amino acid residues and shares 99% identity with the human orthologue in the CRD (3, 4). Frizzled-7 mRNA has been detected in relatively large amounts in adult skeletal muscle, plancenta and heart, and fetal kidney and lung (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Frizzled-7 can activate canonical Wnt/ beta -Catenin signaling, as well as a non-canonical pathway involved in tissue morphogenesis (4, 5).

References

Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
Hsieh, J-C. (2004) Front. Biosci. 9:1333.
Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
Sheldahl, L.C. et al. (1999) Curr. Biol. 9:695.
Medina, A. et al. (2000) Mech. Dev. 92:227.

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7