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Mouse Frizzled-9 Antibody

Catalog #: AF2440
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2440
AF2440-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-9 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Frizzled-9
Leu24-Asp186
Accession # Q9R216
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Frizzled‑9
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of embryonic mouse brain (E11)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-9

Frizzled-9 belongs to the Frizzled family of seven-transmembrane receptors, which are signaling receptors for the family of secreted Wnt ligands. The amino-terminal extracellular cysteine-rich domains of human and mouse Frizzled-9 share 94% amino acid sequence homology.

Entrez Gene IDs
8326 (Human); 14371 (Mouse)
Alternate Names
CD349 antigen; CD349; frizzled (Drosophila) homolog 9; frizzled homolog 9 (Drosophila); Frizzled9; Frizzled-9; fz-9; FZD3fzE6; FZD9; FzE6; hFz9

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Citations for Mouse Frizzled-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse
    Authors: JM Collinson, NO Lindström, C Neves, K Wallace, C Meharg, RH Charles, ZK Ross, AM Fraser, I Mbogo, K Oras, M Nakamoto, S Barker, S Duce, Z Miedzybrod, N Vargesson
    Development, 2018-02-08;145(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Applications: Bioassay

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