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Mouse Galectin-1 Antibody

Catalog #: AF1245
22 Citations 5 Reviews Datasheet / COA / SDS
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AF1245
AF1245-SP
Galectin‑1 in Mouse Thymus.
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Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Galectin-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin‑1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin‑8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Galectin-1
Ala2-Glu135
Accession # P16045
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
THP‑1 human acute monocytic leukemia cell line, L6 rat myoblast cell line, C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line and Mouse Thymus
Simple Western
5 µg/mL
L6 rat myoblast cell line, C2C12 mouse myoblast cell line, THP‑1 human acute monocytic leukemia cell line and Neuro‑2A mouse neuroblastoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Mouse Galectin-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Galectin‑1 Biotinylated Antibody (Catalog # BAF1245)

Standard: Recombinant Mouse Galectin-1 Protein (Catalog # 1245-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Galectin‑1 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Galectin‑1 in Mouse Thymus. Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of Galectin-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Galectin‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western View Larger

Detection of Mouse Galectin‑1 by Simple WesternTM. Simple Western lane view shows lysates of L6 rat myoblast cell line, C2C12 mouse myoblast cell line, THP‑1 human acute monocytic leukemia cell line and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Western Blot View Larger

Detection of Mouse Galectin‑1 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, L6 rat myoblast cell line, C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line and Mouse Thymus. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).

References
  1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2001) Biochimie 83:667.
  4. R&D Systems’ Cytokine Bulletin, Summer (2002).
  5. Goldring, K. et al. (2002) J. Cell Science 115:355.
Entrez Gene IDs
3956 (Human); 16852 (Mouse)
Alternate Names
14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1

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Citations for Mouse Galectin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Vaccination against galectin-1 promotes cytotoxic T-cell infiltration in melanoma and reduces tumor burden
    Authors: Julia Femel, Luuk van Hooren, Melanie Herre, Jessica Cedervall, Falk Saupe, Elisabeth J. M. Huijbers et al.
    Cancer Immunology, Immunotherapy
  2. Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits
    Authors: LG Morosi, AM Cutine, AJ Cagnoni, MN Manselle-C, DO Croci, JP Merlo, RM Morales, M May, JM Pérez-Sáez, MR Girotti, SP Méndez-Hue, B Pucci, AH Gil, SP Huernos, GH Docena, AM Sambuelli, MA Toscano, GA Rabinovich, KV Mariño
    Science Advances, 2021-06-18;7(25):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  3. Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model
    Authors: V Baloche, J Rivière, TBT Tran, A Gelin, O Bawa, N Signolle, MBK Diop, P Dessen, S Beq, M David, P Busson
    Scientific Reports, 2021-03-04;11(1):5227.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche
    Authors: JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda
    Stem Cell Res Ther, 2020-09-29;11(1):425.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. A Pro-resolving Role for Galectin-1 in Acute Inflammation
    Authors: HL Law, RD Wright, AJ Iqbal, LV Norling, D Cooper
    Front Pharmacol, 2020-03-20;11(0):274.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Galectin-1–driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance
    Authors: Dhanya K. Nambiar, Todd Aguilera, Hongbin Cao, Shirley Kwok, Christina Kong, Joshua Bloomstein et al.
    Journal of Clinical Investigation
  7. Galectins-1 and-3 Increase in Equine Post-traumatic Osteoarthritis
    Authors: Heidi L. Reesink, Alan J. Nixon, Jin Su, Sherry Liu, Ryan M. Sutton, Sabine Mann et al.
    Frontiers in Veterinary Science
  8. Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics
    Authors: MS Tsai, MT Chiang, DL Tsai, CW Yang, HS Hou, YR Li, PC Chang, HH Lin, HY Chen, IS Hwang, PK Wei, CP Hsu, KI Lin, FT Liu, LY Chau
    Sci Rep, 2018-07-31;8(1):11497.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, Western Blot
  9. Galectin-1 promotes an M2 macrophage response to polydioxanone scaffolds
    Authors: Daniel Abebayehu, Andrew Spence, Barbara D. Boyan, Zvi Schwartz, John J. Ryan, Michael J. McClure
    Journal of Biomedical Materials Research Part A
  10. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    Exp. Eye Res, 2017-02-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Galectin-1 in injured rat spinal cord: Implications for macrophage phagocytosis and neural repair
    Authors: Andrew D. Gaudet, David R. Sweet, Nicole K. Polinski, Zhen Guan, Phillip G. Popovich
    Molecular and Cellular Neuroscience
  12. Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair.
    Authors: Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, Jaiswal J
    Nat Commun, 2014-12-23;5(0):5646.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity.
    Authors: Baker G, Chockley P, Yadav V, Doherty R, Ritt M, Sivaramakrishnan S, Castro M, Lowenstein P
    Cancer Res, 2014-07-18;74(18):5079-90.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  14. Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion.
    Authors: Szebeni G, Kriston-Pal E, Blazso P, Katona R, Novak J, Szabo E, Czibula A, Fajka-Boja R, Hegyi B, Uher F, Krenacs L, Joo G, Monostori E
    PLoS ONE, 2012-07-23;7(7):e41372.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Impaired spatial and contextual memory formation in galectin-1 deficient mice.
    Mol Brain, 2011-09-01;4(0):33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
    Authors: Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
    Am. J. Pathol., 2011-03-01;178(3):1201-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  17. Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
    Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
    J. Neurochem., 2010-12-02;116(2):217-26.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome.
    Authors: Shimizu M, Khoshnoodi J, Akimoto Y, Kawakami H, Hirano H, Higashihara E, Hosoyamada M, Sekine Y, Kurayama R, Kurayama H, Joh K, Hirabayashi J, Kasai K, Tryggvason K, Ito N, Yan K
    Lab. Invest., 2008-12-15;89(2):178-95.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells.
    Authors: Plachta N, Annaheim C, Bissiere S, Lin S, Ruegg M, Hoving S, Muller D, Poirier F, Bibel M, Barde YA
    Nat. Neurosci., 2007-05-07;10(6):712-9.
    Species: Mouse, Rat
    Sample Types: In Vivo, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Neutralization
  20. Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
    Authors: Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
    Am. J. Pathol., 2007-02-01;170(2):546-56.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  21. Galectin-1 supports survival of naive T cells without promoting cell proliferation.
    Authors: Endharti AT, Zhou YW, Nakashima I, Suzuki H
    Eur. J. Immunol., 2005-01-01;35(1):86-97.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  22. Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation
    Authors: Chiang MT, Chen IM, Hsu FF et al.
    Arterioscler Thromb Vasc Biol

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Mouse Galectin-1 Antibody
By Anonymous on 08/17/2016
Application: WB Sample Tested: U-87 MG human glioblastoma/astrocytoma cell line Species: Mouse

1:2000 dilution in TBS-Tween 20 + 5% BSA

Western Blot Mouse Galectin-1 Antibody AF1245
Mouse Galectin‑1 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cell Lysates Species: Human

The antibody is very specific at 1:1000 and also at 1:2500 dilutions.
Specificity: Specific
Sensitivity: Sensitive
Buffer: BSA
Dilution: 1:1000

Western Blot Mouse Galectin‑1 Antibody AF1245
Mouse Galectin‑1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Mouse

The antibody specifically detects the stromal galectin-1 in normal tissues.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000

Immunohistochemistry Mouse Galectin‑1 Antibody AF1245
Mouse Galectin‑1 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human fibroblast Species: Human

These are HFF-2 cells that show the staining for this antibody.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS`
Dilution: 1:500

Immunocytochemistry/Immunofluorescence Mouse Galectin‑1 Antibody AF1245
Mouse Galectin‑1 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cancer Cells Species: Human

Very specific in detection of galectin-1 in cancer cells.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000

Immunocytochemistry/Immunofluorescence Mouse Galectin‑1 Antibody AF1245