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Mouse GDF-3 Antibody

Catalog #: MAB953 Datasheet / COA / SDS
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MAB953
MAB953-SP
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Mouse GDF-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse GDF-1, -5, -6, -7, -8, or -9 is observed.
Source
Monoclonal Rat IgG2A Clone # 144728
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse GDF-3
Ala253-Gly366
Accession # Q07104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse GDF‑3 (Catalog # 958-G3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-3

GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, adipose tissue and the brain (2‑4). In ES cells, it maintains pluripotency and influences early cell fate decisions (5, 6). For example, frog embryos injected with GDF-3 develop a secondary dorsal axis and deletion of mouse GDF-3 can produce defects in the anterior visceral endoderm of the pre‑gastrulation embryo (5, 6). In adipocytes, GDF-3 is induced by a high fat diet and promotes adipogenesis (3). GDF-3 has been reported to oppose BMP’s functions and to have a nodal-like activity in early development (1). The 366 amino acid (aa) mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein that contains one potential N-glycosylation site. Most of GDF-3 is present as the prepro form, while the mature GDF-3 is presumably the secreted, active form (1). The mature protein contains the cysteine-knot structure that is conserved throughout family members. Since it lacks the fourth cysteine, which is responsible for the formation of inter-molecular disulfide bond, GDF-3 may exist as a non-covalent homodimer. Mature mouse GDF-3 shares 90%, 83% and 83% aa identity with rat, human and canine GDF-3, respectively. Among family members, mature GDF-3 is most similar to mouse BMP-6 (45% aa identity) and Xenopus VG-1 (52% aa identity).

References
  1. Levine, A. J. and A. H. Brivanlou (2006) Cell Cycle 5:1069.
  2. McPherron, A. C. and S-J. Lee (1993) J. Biol. Chem. 268:3444.
  3. Wang, W. et al. (2004) Biochem. Biophys. Res. Comm. 321:1024.
  4. Hexige, S. et al. (2005) Neurosci. Lett. 389:83.
  5. Levine, A. J. and A. H. Brivanlou (2005) Development 133:209.
  6. Chen, C. et al. (2006) Development 133:319.
Long Name
Growth Differentiation Factor 3
Entrez Gene IDs
9573 (Human); 14562 (Mouse)
Alternate Names
GDF3; GDF-3; growth differentiation factor 3; growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; Vgr-2

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