Mouse GDF-8/Myostatin Propeptide Antibody

Catalog #: MAB7881
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7881
MAB7881-SP

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Citations (1)

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Mouse GDF-8/Myostatin Propeptide Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GDF-8 propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GDF‑1 propeptide, rmGDF-3 propeptide, rmGDF-5, or rmGDF-6 is observed.

Source

Monoclonal Rat IgG_2B Clone # 84231

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GDF-8
Asn25-Ser376
Accession # O08689

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse GDF‑8/Myostatin Propeptide (Catalog # 1539-PG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

References

McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
McPherron, A.C. et al. (1997) Nature 387:83.
Zimmers, T.A. et al. (2002) Science 296:1486.
Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
McFarlane, C. et al. (2005) Dev. Biol. 283:58.
Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
Thies, R.S. et al. (2001) Growth Factors 18:251.
Amthor, H. et al. (2004) Dev. Biol. 270:19.
Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
Guo, T. et al. (2009) PloS ONE 4:e4937.

Long Name

Growth Differentiation Factor 8

Entrez Gene IDs

2660 (Human); 17700 (Mouse)

Alternate Names

GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin

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Citation for Mouse GDF-8/Myostatin Propeptide Antibody

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