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Mouse gp130 Antibody

Catalog #: MAB4681
10 Citations Datasheet / COA / SDS
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MAB4681
MAB4681-SP
Detection of gp130 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse gp130 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.
Source
Monoclonal Rat IgG2A Clone # 125623
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse gp130
Gln23-Glu617 (predicted)
Accession # Q6PDI9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of gp130 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of gp130 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry gp130 antibody in M1 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

gp130 in M1 Mouse Cell Line. gp130 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013)and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Detection of Mouse gp130/CD130 by Immunohistochemistry View Larger

Detection of Mouse gp130/CD130 by Immunohistochemistry IL-6 deficiency leads to decreased gp130 protein in the retina. (A) Fold difference of Gp130 mRNA in the IL-6−/− retina normalized to WT Gp130 mRNA (dotted line) via the delta delta Ct method. (B) Densitometry analysis (right) of gp130 and beta -actin immunoblotting (left) in WT (white) and IL-6−/− (gray) retina.; student’s t-test. Error bars indicates STDEV. (C–D) Whole mount (C) and sagittal cross sections (D) of WT (left) and IL-6−/− (right) retina reveal co-localization (arrowheads; C and arrows; D) of gp130 immunolabeling (green) with beta -Tubulin+ RGCs. Error bars represent standard deviation and asterisks indicate p<0.05 (A,B). Scale bars = 20 μm (C,D). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27747134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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Citations for Mouse gp130 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Stressor-dependent Alterations in Glycoprotein 130: Implications for Glial Cell Reactivity, Cytokine Signaling and Ganglion Cell Health in Glaucoma
    Authors: FD Echevarria, CC Walker, SK Abella, M Won, RM Sappington
    J Clin Exp Ophthalmol
  2. Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130
    Authors: AN Wilkinson, KH Gartlan, G Kelly, LD Samson, SD Olver, J Avery, N Zomerdijk, SK Tey, JS Lee, S Vuckovic, GR Hill
    J. Immunol., 2018-04-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling
    Authors: S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat
    J. Biol. Chem, 2017-03-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Dendritic cell MST1 inhibits Th17 differentiation
    Authors: C Li, Y Bi, Y Li, H Yang, Q Yu, J Wang, Y Wang, H Su, A Jia, Y Hu, L Han, J Zhang, S Li, W Tao, G Liu
    Nat Commun, 2017-02-01;8(0):14275.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts.
    Authors: Le Goff B, Singbrant S, Tonkin B, Martin T, Romas E, Sims N, Walsh N
    Cytokine, 2014-04-22;68(2):101-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Ifitm3 limits the severity of acute influenza in mice.
    Authors: Bailey, Charles, Huang, I-Chueh, Kam, Christin, Farzan, Michael
    PLoS Pathog, 2012-09-06;8(9):e1002909.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model.
    Authors: Matsumoto S, Hara T, Mitsuyama K, Yamamoto M, Tsuruta O, Sata M, Scheller J, Rose-John S, Kado S, Takada T
    J. Immunol., 2009-12-30;184(3):1543-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. TH and NPY in sympathetic neurovascular cultures: role of LIF and NT-3.
    Am. J. Physiol., Cell Physiol., 2007-11-21;294(1):C306-12.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Interleukin-6: A Constitutive Modulator of Glycoprotein 130, Neuroinflammatory and Cell Survival Signaling in Retina
    Authors: Franklin D. Echevarria, Abigayle E. Rickman, Rebecca M. Sappington
    Journal of Clinical & Cellular Immunology
  10. Leukemia Inhibitory Factor Represses GnRH Gene Expression via cFOS during Inflammation in Male Mice
    Authors: Nancy M. Lainez, Djurdjica Coss
    Neuroendocrinology

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