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Mouse HB-EGF Antibody

Catalog #: AF8239
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8239
AF8239-SP
Detection of Mouse HB‑EGF by Western Blot.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse HB-EGF Antibody Summary

Species Reactivity
Mouse
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse HB-EGF
Asp63-Leu148
Accession # Q06186
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse HB-EGF antibody by Western Blot. View Larger

Detection of Mouse HB‑EGF by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HB-EGF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry HB-EGF antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

HB‑EGF in Mouse Embryo. HB-EGF was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HB-EGF

Heparin-binding EGF-like growth factor, HB-EGF, is a 19-23 kDa glycoprotein, member of the EGF family of mitogens that exists in multiple forms as a result of heterogenous O-glycosylation.  HB-EGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function and promotes smooth muscle cell proliferation. HB-EGF may be involved in macrophage-mediated cellular proliferation. The cDNA for mouse Proheparin-binding EGF-like growth factor encodes a 208 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB‑EGF. Like EGF, TGF-alpha, and AR, HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF.

Long Name
Heparin Binding EGF-like Growth Factor
Entrez Gene IDs
1839 (Human); 15200 (Mouse)
Alternate Names
diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor

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Citations for Mouse HB-EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Poly(ester amide) particles for controlled delivery of celecoxib
    Authors: Ian J. Villamagna, Trent N. Gordon, Mark B. Hurtig, Frank Beier, Elizabeth R. Gillies
    Journal of Biomedical Materials Research Part A
  2. EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression
    Authors: Yulong Wei, Xiaoyuan Ma, Hao Sun, Tao Gui, Jun Li, Lutian Yao et al.
    Journal of Bone and Mineral Research
  3. Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation
    Authors: L Zhou, W Zhou, AM Joseph, C Chu, GG Putzel, B Fang, F Teng, M Lyu, H Yano, KI Andreasson, E Mekada, G Eberl, GF Sonnenberg
    Nature Immunology, 2022-01-31;23(2):251-261.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Intranasal delivery of VEGF enhances compensatory lung growth in mice
    Authors: DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder
    PLoS ONE, 2018-06-07;13(6):e0198700.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Ablation of peri-insult generated granule cells after epilepsy onset halts disease progression
    Authors: BE Hosford, S Rowley, JP Liska, SC Danzer
    Sci Rep, 2017-12-21;7(1):18015.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Ablation of Newly Generated Hippocampal Granule Cells Has Disease-Modifying Effects in Epilepsy
    Authors: Steve C Danzer
    J. Neurosci., 2016-10-26;36(43):11013-11023.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. MicroRNA-132 enhances transition from inflammation to proliferation during wound healing.
    Authors: Li D, Wang A, Liu X, Meisgen F, Grunler J, Botusan I, Narayanan S, Erikci E, Li X, Blomqvist L, DU L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina S, Stahle M, Landen N
    J Clin Invest, 2015-06-29;125(8):3008-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Single nuclei RNA-seq of mouse placental labyrinth development
    Authors: Bryan Marsh, Robert Blelloch
    eLife
  9. Targeting cartilage EGFR pathway for osteoarthritis treatment
    Authors: Yulong Wei, Lijun Luo, Tao Gui, Feifan Yu, Lesan Yan, Lutian Yao et al.
    Science Translational Medicine

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