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Mouse Hemopexin Antibody

Catalog #: AF7007
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7007
AF7007-SP
Detection of Mouse Hemopexin by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Hemopexin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Hemopexin in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant human Hemopexin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Hemopexin
Ser24-Gln460 (His30Asn)
Accession # Q91X72
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Hemopexin antibody by Western Blot. View Larger

Detection of Mouse Hemopexin by Western Blot. Western blot shows mouse plasma and lysates of mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse Hemopexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hemopexin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Hemopexin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Hemopexin in Mouse Kidney. Hemopexin was detected in immersion fixed frozen sections of adult mouse kidney using Sheep Anti-Mouse Hemopexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7007) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to tubule epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemopexin

HPX (Hemopexin) is a 60-70 kDa secreted member of the hemopexin family of molecules. Although it is principally expressed by hepatocytes, it is also reportedly secreted by macrophages, neurons, astrocytes, renal mesangial cells and fibroblasts. It is considered an acute phase protein, and demonstrates a number of effects. Principally, it binds free and albumin-bound heme, neutralizing its potential for oxidative damage to cell membranes. Heme is removed from HPX internally following binding of the HPX:heme complex to CD91 on hepatocytes and macrophages. HPX also dampens the proinflammatory response of macrophages to TLR2 and TLR4 activation. And HPX also shows serine protease activity which may impact cell surface proteins and ECM. Mature mouse HPX is a 437 amino acid (aa) glycoprotein (aa 24-460). It contains five consecutive HPX-like domains (aa 56-349) that likely impart binding-partner specificity to HPX. There are multiple potential isoform variants. One shows a two aa substitution for aa 134-460, a second contains a deletion of aa 60-100, a third possesses a 14 aa substitution for aa 235-460, while a fourth shows a two aa substitution for aa 153-460. Mature mouse HPX shares 92% and 77% aa identity with rat and human HPX, respectively.

Entrez Gene IDs
3263 (Human); 15458 (Mouse); 58917 (Rat)
Alternate Names
Beta-1B-glycoprotein; FLJ56652; Hemopexin; HPX; HX

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Citations for Mouse Hemopexin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice
    Authors: J Liu, S Lane, R Lall, M Russo, L Farrell, M Debreli Co, C Curtin, R Araujo-Gut, M Scherrer-C, BH Trachtenbe, J Kim, E Tolosano, A Ghigo, RE Gerszten, A Asnani
    Science Advances, 2022-12-23;8(51):eadc9245.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Western Blot
  2. Flavin-Containing Monooxygenase 3 (FMO3) Is Critical for Dioxin-Induced Reorganization of the Gut Microbiome and Host Insulin Sensitivity
    Authors: W Massey, LJ Osborn, R Banerjee, A Horak, KK Fung, D Orabi, ER Chan, N Sangwan, Z Wang, JM Brown
    Metabolites, 2022-04-18;12(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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