Mouse HGF Biotinylated Antibody

Catalog #: BAF2207
1 Citation Datasheet / COA / SDS

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BAF2207

All HGF Antibodies

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Mouse HGF Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) HGF, rhHGF R, rmHGF R, rhHGF-A, and rmHGF-A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse HGF
Gln33-Leu728
Accession # Q53WS5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse HGF (Catalog # 2207-HG)

Mouse HGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse HGF Antibody (Catalog # AF2207)

Standard: Recombinant Mouse HGF Protein (Catalog # 2207-HG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References

Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
Corso, S. et al. (2005) Trends Mol. Med. 11:284.
Maeshima, A. et al. (2000) Kid. Int. 58:1511.
Montesano, R. et al. (1991) Cell 67:901.
Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
Mineo, R. et al. (1994) Endocrinology 145:4355.
Urbanek, K. et al. (2005) Circ. Res. 97:663.

Long Name

Hepatocyte Growth Factor

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Alternate Names

deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

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Citation for Mouse HGF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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