Mouse HVEM/TNFRSF14 Antibody

Catalog #: MAB2516 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB2516-100
MAB2516-SP

Detection of HVEM/TNFRSF14 in Mouse Splenocytes by Flow Cytometry.

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Mouse HVEM/TNFRSF14 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HVEM/TNFRSF14 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2024B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse HVEM/TNFRSF14
Gln39-Val207
Accession # NP_849262

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of HVEM/TNFRSF14 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rabbit Anti-Mouse HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB2516, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of HVEM/TNFRSF14 in CHO Chinese Hamster Cell Line Transfected with Mouse HVEM by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse HVEM was stained with Rabbit Anti-Mouse HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB2516, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator) is a type I membrane protein that is TNF receptor superfamily member 14 (TNFRSF14) (1). The mouse HVEM cDNA encodes a 275 amino acid (aa) protein. It contains a 36 aa signal peptide, a 170 aa extracellular domain with three cysteine rich domains (CRD), a 24 aa transmembrane region and a 45 aa cytoplasmic tail with a TRAF interaction domain (1). HVEM expression is highest on naïve, memory and regulatory T cells, but declines during T cell activation (2, 3). It is present at low levels on most resting leukocytes (4). HVEM is a receptor for the IGSF member BTLA (B and T lymphocyte attenuator), CD160, and the TNF family ligand LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) (2, 9). HVEM and BTLA are constitutively expressed on T cells, while LIGHT is generally considered to be inducible upon TCR activation. In the absence of activation, HVEM and BTLA interact monomerically, either in cis, or in trans. A same cell (or cis) interaction likely promotes general cell survival, while a between cell (or trans) interaction promotes a state of lymphocyte inactivity through the BTLA cytoplasmic domain. Following T cell activation, LIGHT appears and disrupts existing HVEM-BTLA bonds. A LIGHT-HVEM trimer now forms in trans, initiating HVEM-mediated NF kappa B signaling and a proinflammatory response (10). BTLA and LIGHT interactions are not mutually exclusive, but BTLA appears dominant (4, 6, 7). The herpesvirus envelope glycoprotein gD, which binds HVEM CRD1 to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (1, 6, 7, 9). Human, but not mouse, HVEM can also bind lymphotoxin a within CRD2 3 (9, 11). Graft‑vs‑host disease and Th1 type intestinal inflammation can be ameliorated by interrupting T cell LIGHT/HVEM interactions, while disruption of BTLA/HVEM interaction promotes intestinal inflammation (12-14). Mouse HVEM ECD shares 89% and 53% aa identity with rat and human HVEM, respectively. Mouse HVEM can recognize human BTLA and LIGHT, but human HVEM does not recognize mouse ligands (2, 11).

References

Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
Sedy, J.R. et al. (2005) Nat. Immunol. 6:90.
Tao, R. et al. (2008) J. Immunol. 180:6649.
Wang, Y. et al. (2005) J. Clin. Invest. 115:711.
Nelson, C.A. et al. (2008) J. Immunol. 180:940.
Gonzales, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
Cai, G. et al. (2008) Nat. Immunol. 9:176.
Mauri, D.N. et al. (1998) Immunity 8:21.
Ware, C.F. (2008) Immunol. Rev. 223:186.
Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
Xu, Y. et al. (2007) Blood 109:4097.
Wang, J. et al. (2005) J. Immunol. 174:8173.
Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.

Long Name

Herpesvirus Entry Mediator

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Alternate Names

ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.