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Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Catalog #: IC0041N 1 Review Datasheet / COA / SDS
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IC0041N
R&D Systems Antibodies
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Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control Summary

Specificity
Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control
By Harry Hurley on 12/04/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

5 uL of IC0041N added to PBMC stained with BDCA2-PerCP/Cy5.5, CD123-BV510, CD3-PE, CD14-APC/Cy7. Data shown is gated pDC (BDCA2+CD123+).

Flow Cytometry Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control IC0041N