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Mouse IgG2B Fluorescein‑conjugated Control Antibody

Catalog #: IC0041F
8 Citations 1 Review Datasheet / COA / SDS
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IC0041F
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (8)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2B Fluorescein‑conjugated Control Antibody Summary

Specificity
Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4546F, filled histogram) or Mouse IgG Fluorescein -conjugated Isotype Control Antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Mouse IgG2B Fluorescein‑conjugated Control Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo
    Authors: LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider
    Cell Stem Cell, 2019-03-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Porcine Treg depletion with a novel diphtheria toxin-based anti-human CCR4 immunotoxin
    Authors: Zhirui Wang
    Vet. Immunol. Immunopathol., 2016-10-29;182(0):150-158.
    Species: Porcine
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  3. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015-01-01;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  4. Expression, Regulation, and Function of Atypical Chemerin Receptor CCRL2 on Endothelial Cells.
    Authors: Monnier J, Lewen S, O'Hara E, Huang K, Tu H, Butcher EC, Zabel BA
    J. Immunol., 2012-06-13;189(2):956-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  5. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
    Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
    Innate Immun, 2011-11-04;18(3):531-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  6. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011-06-10;187(2):748-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  7. Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission.
    Authors: Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM
    Eur. J. Immunol., 2009-05-01;39(5):1288-300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  8. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005-09-15;11(18):6686-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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Mouse IgG2B Fluorescein‑conjugated Control Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human