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Mouse IgG2B Isotype Control

Catalog #: MAB0042
7 Citations 1 Review Datasheet / COA / SDS
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MAB0042
R&D Systems Antibodies
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Citations (7)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2B Isotype Control Summary

Specificity
Recognizes the E-tag peptide. No background staining was detected by immunohistochemistry on a variety of tissues.
Source
Monoclonal Mouse IgG2B Clone # 73009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant protein with a C-terminal E-tag (aa sequence GAPVPYPDPLEPR)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Control
Negative control for immunohistochemistry or immunocytochemistry experiments. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Mouse IgG2B Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Humanized anti-DEspR IgG4S228P antibody increases overall survival in a pancreatic cancer stem cell-xenograft peritoneal carcinomatosis ratnu/nu model
    Authors: CM Gromisch, GLA Tan, KA Pasion, AM Moran, MS Gromisch, MW Grinstaff, FJ Carr, VLM Herrera, N Ruiz-Opazo
    BMC Cancer, 2021-04-14;21(1):407.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
    PLoS ONE, 2014-08-20;9(8):e105375.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Isotype Control
  3. Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
    Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
    Clin. Exp. Allergy, 2011-06-16;41(10):1392-1399.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Control
  4. S100A11, an dual mediator for growth regulation of human keratinocytes.
    Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
    Mol. Biol. Cell, 2007-10-31;19(1):78-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  5. Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
    Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-30;293(1):L142-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Control
  6. Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
    Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
    J. Virol., 2007-03-21;81(11):5882-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  7. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006-12-01;169(6):2094-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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Reviews for Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control
By Anonymous on 05/20/2018
Application: B/N Sample Tested: Breast cancer cells Species: Human

We used this as an Isotype control for our Endothelial Chemotaxis Assay experiments. Conditioned Medium from Breast cancer cells mixed with IgG2B Isotype Control was used to compare Conditioned medium containing cytokines (IL6, IL8, IL1B)
and VEGF-A (IgG2B) neutralization antibody cocktail.
Cancer Res; 78(5) March 1, 2018 (Fig 6F)

Block/Neutralize Mouse IgG2B Isotype Control MAB0042