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Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Catalog #: MLA00
32 Citations 6 Reviews Datasheet / COA / SDS
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MLA00
Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve
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Product Details
Procedure
Citations (32)
FAQs
Supplemental Products
Reviews (6)
Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL)
Sensitivity
2.5 pg/mL
Assay Range
4.7 - 300 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.

Recovery

The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 102 92-109
Serum (n=6) 94 88-103

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-1 alpha/IL-1F1 ELISA Linearity

Scientific Data

N/A Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve View Larger

Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names:
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. The cGAS-STING-YY1 axis accelerates progression of neurodegeneration in a mouse model of Parkinson's disease via LCN2-dependent astrocyte senescence
    Authors: Jiang, SY;Tian, T;Yao, H;Xia, XM;Wang, C;Cao, L;Hu, G;Du, RH;Lu, M;
    Cell death and differentiation
    Species: Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  2. The interaction between aging and protein malnutrition modulates peritoneal macrophage function: An experimental study in male mice
    Authors: GK Vivian, RO da Silva, ACA Santos, AA Hastreiter, CC Dias, EN Makiyama, P Borelli, C de Oliveir, RA Fock
    Experimental gerontology, 2022-11-11;171(0):112025.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies
    Authors: M Kamiya, F Mizoguchi, K Kawahata, D Wang, M Nishibori, J Day, C Louis, IP Wicks, H Kohsaka, S Yasuda
    Nature Communications, 2022-01-10;13(1):166.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors
    Authors: P Innamarato, J Morse, A Mackay, S Asby, M Beatty, J Blauvelt, S Kidd, JE Mullinax, AA Sarnaik, S Pilon-Thom
    BMC Cancer, 2021-06-30;21(1):756.
    Species: Mouse
    Sample Types: Serum
  5. Augmented secretion of IL-1&alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells
    Authors: A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh
    Journal of oral biosciences, 2021-06-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Type 1 interferon mediates chronic stress-induced neuroinflammation and behavioral deficits via complement component 3-dependent pathway
    Authors: A Tripathi, C Whitehead, K Surrao, A Pillai, A Madeshiya, Y Li, H Khodadadi, AO Ahmed, G Turecki, B Baban, A Pillai
    Molecular Psychiatry, 2021-04-08;0(0):.
    Species: Mouse
    Sample Types: Serum
  7. Pancreatic, but not myeloid-cell, expression of interleukin-1alpha is required for maintenance of insulin secretion and whole body glucose homeostasis
    Authors: JJ Collier, HM Batdorf, TM Martin, KE Rohli, DH Burk, D Lu, CR Cooley, MD Karlstad, JW Jackson, TE Sparer, J Zhang, RL Mynatt, SJ Burke
    Molecular Metabolism, 2020-12-05;44(0):101140.
    Species: Mouse
    Sample Types: Cell Lysates
  8. Impact of combined ultrasound-microwave treatment on structural and functional properties of golden threadfin bream (Nemipterus virgatus) myofibrillar proteins and hydrolysates
    Authors: Z Li, J Wang, B Zheng, Z Guo
    Ultrason Sonochem, 2020-03-09;65(0):105063.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease
    Authors: IJ Yeo, MJ Lee, A Baek, Z Miller, D Bhattarai, YM Baek, HJ Jeong, YK Kim, DE Kim, JT Hong, KB Kim
    Sci Rep, 2019-12-05;9(1):18393.
    Species: Mouse
    Sample Types: Serum
  10. Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice
    Authors: N Guo, V DeAngelis, C Zhu, EH Schuchman, CM Simonaro
    JIMD Rep, 2018-04-14;0(0):.
    Species: Mouse
    Sample Types: Plasma
  11. Specific calpain inhibition protects kidney against inflammaging
    Authors: G Hanouna, L Mesnard, S Vandermeer, J Perez, S Placier, JP Haymann, F Campagne, J Moroch, A Bataille, L Baud, E Letavernie
    Sci Rep, 2017-08-14;7(1):8016.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  12. Kruppel-like factor 6 promotes macrophage-mediated inflammation by suppressing B cell leukemia/lymphoma 6 expression
    J Biol Chem, 2016-08-18;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
    Authors: Meng L, Jin W, Wang X
    Proc Natl Acad Sci U S A, 2015-08-17;112(35):11007-12.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Pivotal role of phospholipase D1 in tumor necrosis factor-alpha-mediated inflammation and scar formation after myocardial ischemia and reperfusion in mice.
    Authors: Schonberger T, Jurgens T, Muller J, Armbruster N, Niermann C, Gorressen S, Sommer J, Tian H, di Paolo G, Scheller J, Fischer J, Gawaz M, Elvers M
    Am J Pathol, 2014-07-18;184(9):2450-64.
    Species: Mouse
    Sample Types: Plasma
  15. The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
    Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
    Cytokine, 2014-07-06;69(2):218-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Running exercise alleviates trabecular bone loss and osteopenia in hemizygous beta-globin knockout thalassemic mice.
    Authors: Thongchote K, Svasti S, Teerapornpuntakit J, Krishnamra N, Charoenphandhu N
    Am J Physiol Endocrinol Metab, 2014-04-29;306(12):E1406-17.
    Species: Mouse
    Sample Types: Serum
  17. The senescence-associated secretory phenotype promotes benign prostatic hyperplasia.
    Authors: Vital P, Castro P, Tsang S, Ittmann M
    Am J Pathol, 2014-01-13;184(3):721-31.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Tissue-specific opposing functions of the inflammasome adaptor ASC in the regulation of epithelial skin carcinogenesis.
    Authors: Drexler, Stefan K, Bonsignore, Luca, Masin, Mark, Tardivel, Aubry, Jackstadt, Rene, Hermeking, Heiko, Schneider, Pascal, Gross, Olaf, Tschopp, Jurg, Yazdi, Amir S
    Proc Natl Acad Sci U S A, 2012-10-22;109(45):18384-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
    Blood, 2012-07-17;120(13):2600-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Sphingosine regulates the NLRP3-inflammasome and IL-1beta release from macrophages.
    Authors: Luheshi NM, Giles JA, Lopez-Castejon G, Brough D
    Eur. J. Immunol., 2011-12-27;42(3):716-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
    Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
    Eur. Respir. J., 2011-05-26;38(5):1019-28.
    Species: Mouse
    Sample Types: BALF
  22. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
    Authors: Wang H, Cheng F
    J. Immunol., 2011-03-02;186(0):3986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
    Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
    Nat. Immunol., 2010-09-12;11(10):897-904.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
    Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
    Cytokine, 2009-12-29;49(3):256-63.
    Species: Mouse
    Sample Types: BALF
  25. Tumor necrosis factor-alpha and interleukin-1 antagonists alleviate inflammatory skin changes associated with epidermal growth factor receptor antibody therapy in mice.
    Authors: Surguladze D, Deevi D, Claros N, Corcoran E, Wang S, Plym MJ, Wu Y, Doody J, Mauro DJ, Witte L, Busam KJ, Pytowski B, Rodeck U, Tonra JR
    Cancer Res., 2009-07-07;69(14):5643-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
    Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
    J. Immunol., 2009-06-19;183(2):1291-300.
    Species: Mouse
    Sample Types: BALF
  27. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2006-10-18;81(2):783-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. Absence of IL-1 receptor antagonist impaired wound healing along with aberrant NF-kappaB activation and a reciprocal suppression of TGF-beta signal pathway.
    Authors: Ishida Y, Kondo T, Kimura A, Matsushima K, Mukaida N
    J. Immunol., 2006-05-01;176(9):5598-606.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. IL-1 mediates TNF-induced osteoclastogenesis.
    Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
    J. Clin. Invest., 2005-02-01;115(2):282-90.
    Species: Mouse
    Sample Types: Cell Lysates
  30. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411.
    Species: Mouse
    Sample Types: Serum
  31. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis.
    Authors: Hao Z, Hampel B, Yagita H, Rajewsky K
    J. Exp. Med., 2004-05-17;199(10):1355-65.
    Species: Mouse
    Sample Types: Serum
  32. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001-07-01;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Average Rating: 4.8 (Based on 6 Reviews)

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Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 12/22/2021
Sample Tested: bone marrow

5 times dilution

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 12/21/2021
Sample Tested: moues cell line conditioned media
Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 05/21/2021
Sample Tested: Bone marrow lavage,EDTA Plasma
Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 08/06/2018
Sample Tested: Serum
Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 04/11/2018
Sample Tested: Adipocytes,Pancreatic cancer cells
Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 03/30/2018
Sample Tested: mouse pancreatic cell line