Mouse IL-11 Antibody

Catalog #: AF-418-NA
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-418-NA
AF-418-SP

Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 Antibody.

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All IL-11 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-11 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-11 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL-11
Gly23-Leu199
Accession # P47873

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑11 (Catalog # 418-ML)

Neutralization

Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line [Nordan, R.P. et al. (1987) J. Immunol. 139:813]. The Neutralization Dose (ND₅₀) is typically 8-50 ng/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑11.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 Antibody. Recombinant Mouse IL-11 (418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-11 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-418-NA). The ND₅₀ is typically 8-50 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11

Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The mouse IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 22 amino acid residue hydrophobic signal that is processed proteolytically to generate the 177 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites.

IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4, and SCF to shorten the G₀ period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cells. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute-phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor alpha chain and gp130.

Long Name

Interleukin 11

Entrez Gene IDs

3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)

Alternate Names

Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

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Related Research Areas

Citations for Mouse IL-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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